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pET28_Caspase-4_K356D

Plasmid

#214312

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

K356D

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-TRBP

Plasmid

#50351

Purpose

bacterial expression of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

TRBP (TARBP2 Human)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1delltaH4

Plasmid

#71713

Purpose

Membrane scaffold protein (MSP), helix 4 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pET28b-FOXP2_P6

Plasmid

#122649

Purpose

Expresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-mNG-NL

Plasmid

#169106

Purpose

Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - mNeonGreen - NanoLuc

Use

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1E3D1

Plasmid

#199772

Purpose

Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

Insert

cMSP1E3D1

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Extended cMSP1D1; external His-Tags flanking the …

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_T5-ARG1

Plasmid

#106476

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T5

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-PFO

Plasmid

#92109

Purpose

Recombinant expression in E.Coli

Depositor

Feng Shao

Article

Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

Insert

PFO

Use

Tags

6xHis

Expression

Bacterial

Mutation

Delta27aa

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-SpySrtA

Plasmid

#51139

Purpose

expression plasmid for N-terminal his tagged S.pyogenes SrtA

Depositor

Hidde Ploegh

Article

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.pyogenes SrtA

Use

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-80

Promoter

T7

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH5

Plasmid

#199771

Purpose

Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH5

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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