Skip to main content
Addgene
Showing: 381 - 400 of 1717 results

  1. pFastBac Dual PNG172-STII PLU-HIS

    Plasmid
    #112992
    Purpose
    Baculovirus expression of Drosophila png172-Flag and plu-His
    Depositor
    Terry Orr-Weaver
    Inserts
    png172 (png Fly)
    plu (plu Fly)
    Use
    Tags
    Flag and His
    Expression
    Insect
    Mutation
    png172 mutation
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112992-plasmid-map-sequence-id-217463

    Enlarge

  2. pFastBac Dual PNG-STII PLU-HIS

    Plasmid
    #112993
    Purpose
    Baculovirus expression of Drosophila png-Flag and plu-His
    Depositor
    Terry Orr-Weaver
    Inserts
    png (png Fly)
    plu (plu Fly)
    Use
    Tags
    Flag and His
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    112993-plasmid-map-sequence-id-217440

    Enlarge

  4. pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa

    Plasmid
    #205766
    Purpose
    Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cells
    Depositor
    Edward Boyden
    Article
    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.
    Insert
    JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    205766-plasmid-map-sequence-id-459162

    Enlarge

  7. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

    Plasmid
    #174631
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174631-plasmid-map-sequence-id-346982

    Enlarge

  8. pB80-KIF1A(1-365)-VVDfast-mCherry-SSPB(micro)

    Plasmid
    #174634
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mCherry-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174634-plasmid-map-sequence-id-346991

    Enlarge

  11. pFastBac1 human full-length CENP-E-mNeon-6xHis

    Plasmid
    #180484
    Purpose
    pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells
    Depositor
    Julie Welburn
    Article
    Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1
    Insert
    CENP-E (CENPE human, Human, Synthetic)
    Use
    Tags
    mNeon-6xHis
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    180484-plasmid-map-sequence-id-377471

    Enlarge

  12. pFastBac HTB His-TEV-rDPP9(S729A)-FLAG

    Plasmid
    #217329
    Purpose
    Purification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S729A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217329-plasmid-map-sequence-id-433435

    Enlarge

  13. pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG

    Plasmid
    #217325
    Purpose
    Purification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    N61K
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217325-plasmid-map-sequence-id-438441

    Enlarge

  14. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

    Plasmid
    #217324
    Purpose
    Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217324-plasmid-map-sequence-id-438435

    Enlarge

  15. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

    Plasmid
    #217320
    Purpose
    Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Use
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217320-plasmid-map-sequence-id-438445

    Enlarge

  16. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)

    Plasmid
    #174637
    Purpose
    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
    Use
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174637-plasmid-map-sequence-id-346992

    Enlarge

Showing: 381 - 400 of 1717 results