We narrowed to 161,407 results for: addgene

1. mCherry-Vector

   Plasmid

   #214477

   Purpose

   Vector control for mCherry fusions. Created from the backbone of Addgene #55043 mCherry-Ezrin-N-14 from Michael Davidson.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. DMTF1

   Plasmid

   #36895

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   DMTF1 (DMTF1 Human)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains Myb-like 1 domain, residues 220-274

   Promoter

   Available since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pLIX_403

   Plasmid

   #41395

   Purpose

   Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Plenti-CMV-MCS-RFP-SV-puro

   Plasmid

   #109377

   Purpose

   This plasmid expresses dsRed2 and puromycin resistance

   Depositor

   Jonathan Garlick, Behzad Gerami-Naini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   dsRed2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBbi-pur H-2Kb

   Plasmid

   #111623

   Purpose

   Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

   Depositor

   Jon Yewdell

   Insert

   H2Kb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Neo

   Plasmid

   #127762

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

   Plasmid

   #219826

   Purpose

   Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 29, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

   Plasmid

   #174893

   Purpose

   amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Insert

   mCherry and EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe bleo human PPAR gamma 2

   Plasmid

   #11439

   Depositor

   Ronald Kahn

   Insert

   PPAR gamma 2 (PPARG Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. OTUD5

    Plasmid

    #28270

    Depositor

    Cheryl Arrowsmith

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    OTU DOMAIN (residues 172-339).

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV V5-Nesprin-3

    Plasmid

    #175136

    Purpose

    Lentiviral expression of V5-tagged mouse Syne3

    Depositor

    Larry Gerace

    Insert

    Syne3 (Syne3 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SEC14L3

    Plasmid

    #73240

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SEC14L3 (SEC14L3 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

14. SEC14L4

    Plasmid

    #73233

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SEC14L4 (SEC14L4 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV V5-Sun2

    Plasmid

    #175114

    Purpose

    Lentiviral expression of V5-tagged mouse Sun2

    Depositor

    Larry Gerace

    Insert

    Sun2 (Sun2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. katushka-FMDV-P2A-pTRIP

    Plasmid

    #163522

    Purpose

    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

    Depositor

    Thomas Weber

    Insert

    stopless Katushka-P2A
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PP-G6PI

    Plasmid

    #28283

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-G6PI (PF14_0341 Plasmodium falciparum)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCAGIG-6xHIS.Mm.RPE65-eGFP

    Plasmid

    #41019

    Depositor

    John Nickerson

    Insert

    RPE65 (Rpe65 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPHK1

    Plasmid

    #73260

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLXIN2-RFP

    Plasmid

    #99205

    Purpose

    RFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    Red fluorescent protein
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Tags

    Expression

    Mammalian

    Mutation

    The dsRed cDNA was PCR amplified off AddGene plas…

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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