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We narrowed to 1,118 results for: gibson assembly

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Showing: 381 - 400 of 1118 results

  2. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid
    #216309
    Purpose
    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)
    Depositor
    Mark Howarth
    Publication
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216309-plasmid-map-sequence-id-433372

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  3. pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag

    Plasmid
    #185683
    Purpose
    Low-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.
    Depositor
    Peter Walter
    Publication
    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERN1 (ERN1 Synthetic, Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    (WLLI-GSGS)[359-362]
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    185683-plasmid-map-sequence-id-366421

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  4. pPW3763 : CMVd3-HsIRE1dLKR-HaloTag

    Plasmid
    #185685
    Purpose
    Low-level transient expression of HsIRE1 lacking the linker, kinase, and RNase domains, with a C-terminal HaloTag.
    Depositor
    Peter Walter
    Publication
    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERN1 (ERN1 Synthetic, Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Deletion of linker, kinase, and Rnase domains
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    185685-plasmid-map-sequence-id-366419

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  7. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

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  8. pFP.R265

    Plasmid
    #138167
    Purpose
    Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(VDA).M86.(SDL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138167-plasmid-map-sequence-id-273521

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  9. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

    Plasmid
    #185678
    Purpose
    Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.
    Depositor
    Peter Walter
    Publication
    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERmembrane-HaloTag-KKMP
    Expression
    Mammalian
    Available since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    185678-plasmid-map-sequence-id-366401

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  10. AP1297‐1

    Plasmid
    #87247
    Purpose
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Depositor
    Geraldine Seydoux
    Publication
    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Tags
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…
    Available since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87247-plasmid-map-sequence-id-173228

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  12. pMMACE DEST CMV H2B iRFP

    Plasmid
    #206253
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Publication
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    H2B iRFP713
    Use
    Recombinant baculovirus production, multimate/gat…
    Tags
    iRFP713
    Expression
    Mammalian
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    206253-plasmid-map-sequence-id-406403

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  13. pMMK ENTR 4 CMV GST mTagBFP

    Plasmid
    #206259
    Purpose
    ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.
    Depositor
    Imre Berger
    Publication
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    GTS mTagBFP
    Use
    Multimate/gateway entr 4
    Tags
    BFGT1 (Golgi localisation peptide)
    Expression
    Mammalian
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    206259-plasmid-map-sequence-id-406393

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  14. pMMDS DEST

    Plasmid
    #206264
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.
    Depositor
    Imre Berger
    Publication
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Type
    Empty backbone
    Use
    Cre-donor, multimate/gateway dest vector (multiba…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206264-plasmid-map-sequence-id-406384

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  15. MZB134

    Plasmid
    #228485
    Purpose
    "Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression)
    Depositor
    Richard Baker
    Type
    Empty backbone
    Tags
    6xHis-TEV-Halo-HRV
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228485-plasmid-map-sequence-id-455390

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Showing: 381 - 400 of 1118 results