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pQP-2460
Plasmid#138973PurposeLight-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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mCh-LgBiT-2A-EGFP-Vin-PILATeS
Plasmid#216761PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
Plasmid#216763PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pERB122
Plasmid#58282Purposeconst: miRFKBP5_GFP_IRES_Puro; ind: mC-FRBDepositorInsertsFKBP-targeted miRNA
GFP
Puromycin resistance
inducible mCherry-FRB
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
cytoDCIP
Plasmid#193861PurposeEncodes mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertmCherry-GFP1-10
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CYLBL_Neo_CAGdCas9-KRAB
Plasmid#220439PurposeCAG-dCas9-KRAB knock-in using TALEN at CLYBL locusDepositorInsertdCas9
UseCRISPR and TALENTagsKRABExpressionMammalianMutationD10A and H840APromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
DCIP
Plasmid#193860PurposeEncodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.DepositorInsertNLS-mCherry-GFP1-10
UseTagsSV40 NLSExpressionPlantMutationPromoter35SAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSD080A
Plasmid#218138PurposeLentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.DepositorInsertSD40-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD141B
Plasmid#218139PurposeLentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.DepositorInsertSD56-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD068F
Plasmid#218137PurposeLentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.DepositorInsertSD36-eGFP-IRES2-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRY2(L348F)-CreN
Plasmid#75368PurposeExpresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinaseDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsCre recombinase, residues 19-104ExpressionMammalianMutationL348FPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGImC_Empty
Plasmid#92015PurposeEmpty backbone, includes IRES-mCherryDepositorTypeEmpty backboneUseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
LT3GECIR
Plasmid#111178PurposeTet-ON miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterT3GAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRIS
Plasmid#102584PurposeRegulation of protein localization (near-infrared-blue-light-inducible shuttle)DepositorInsertBphP1-mVenus-CAAX-IRES2-NES-mCherry-QPAS1-AsLOV2cNLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mNG-mCh
Plasmid#124215PurposeCytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mChDepositorInsertmNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only
