We narrowed to 2,326 results for: PUC19

1. pITEdv1

   Plasmid

   #218283

   Purpose

   Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pITEu2

   Plasmid

   #218282

   Purpose

   Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJE13HD7

   Plasmid

   #218281

   Purpose

   Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPAP_scFv_V50A

   Plasmid

   #183734

   Purpose

   Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   Anti-lysozyme scFv
   

   Use

   Tags

   His6-tag

   Expression

   Yeast

   Mutation

   V50A mutation in MFalpha signal

   Promoter

   AOX1 promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

   Plasmid

   #211905

   Purpose

   Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   DARIC-CD19-scFv
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK1

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-N-Solo-sTagRFP-TUBA1B

   Plasmid

   #207765

   Purpose

   Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #199453

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

   Plasmid

   #173211

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ir7a-T2A-QF2 HDR plasmid

   Plasmid

   #140943

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene

   Depositor

   Leslie Vosshall

   Article

   Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

   Insert

   Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTW099

    Plasmid

    #115942

    Purpose

    SGP insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 -98/14 SGP 2x K-turn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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