We narrowed to 5,315 results for: pcdna3.1

1. pcDNA3-HA-human OCRL

   Plasmid

   #22207

   Depositor

   Pietro De Camilli

   Article

   Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

   Insert

   Oculocerebrorenal syndrome of Lowe (OCRL Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HDAC8 Flag

   Plasmid

   #13825

   Depositor

   Eric Verdin

   Article

   Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

   Insert

   HDAC8 (HDAC8 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTE4398

   Plasmid

   #74042

   Purpose

   Expresses human codon-optimized LbCpf1 and Lb crRNA.

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Lb crRNA
   LbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L2125-FRB-GPA-NLuc-CyOFP1

   Plasmid

   #222885

   Purpose

   Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc-10GS-CyOFP1
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SPLICS Po-Mt Short P2A

   Plasmid

   #164116

   Purpose

   Detect the short Peroxisomes-mitochondria contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split GFP Po-Mt Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA SF-HERC2 F6

   Plasmid

   #55789

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA-I-PhoCl-HCVp

   Plasmid

   #87697

   Purpose

   Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
   

   Use

   Tags

   EBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3-SREBP-2

   Plasmid

   #204200

   Purpose

   Mammalian expression of SREBP-2

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-2 (SREBF2 Human)
   

   Use

   Tags

   T7x3

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RA-NES

   Plasmid

   #61019

   Purpose

   ddRFP A-NES

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A
   

   Use

   Tags

   HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CanlonicSF

    Plasmid

    #178342

    Purpose

    To explore lactate dynamics in intact systems

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    CanlonicSF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMV-FlicR1

    Plasmid

    #74142

    Purpose

    Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

    Depositor

    Robert Campbell

    Article

    Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

    Insert

    FlicR1
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 Lats1 (Nigg HS189)

    Plasmid

    #41156

    Depositor

    Erich Nigg

    Article

    Chan et al Oncogene. 2005 Mar 17;24(12):2076-86.

    Insert

    Lats1 (LATS1 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pAL113

    Plasmid

    #22471

    Purpose

    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB NT (PHYB Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    10aaLinker-mCFP-HrasCT

    Expression

    Mammalian

    Mutation

    Y276H

    Promoter

    CMV

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cytosolic FAK biosensor

    Plasmid

    #78300

    Purpose

    FRET biosensor. Visualization of cytosolic FAK activity in live cells

    Depositor

    Yingxiao Wang

    Article

    Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

    Insert

    Cytosolic FAK biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-LiMETER-v2

    Plasmid

    #113934

    Purpose

    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    LOV2 and Stim1 polybasic domain (PB)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-Climp63(1–336)

    Plasmid

    #186957

    Purpose

    Expresses mCherry-Climp63 with partial luminal domain

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Ckap4 (Ckap4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only remain partial luminal domain

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Luc-noPS

    Plasmid

    #177940

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mKO3-TEAD4

    Plasmid

    #221217

    Purpose

    RFP fused TEAD4

    Depositor

    Xiaokun Shu

    Article

    Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

    Insert

    mKO3-TEAD4 (TEAD4 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA SF-HERC2 F4

    Plasmid

    #55787

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
    

    Use

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-BaLgp120-8his

    Plasmid

    #100925

    Purpose

    Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Use

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge