We narrowed to 9,202 results for: sacs

1. pAG415Gal_Gcn4 9acidAGFP

   Plasmid

   #233009

   Purpose

   Galactose iduced expression of Gcn4 9acidAGFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 9acidA
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 ILVtoED WGFP

   Plasmid

   #233007

   Purpose

   Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED W+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG415Gal_Gcn4 solvvol W

   Plasmid

   #232977

   Purpose

   Galactose iduced expression of Gcn4 solvvol W+ in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol W+
   

   Use

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDest17_Gcn4 SATtoG KtoR

   Plasmid

   #231861

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoR

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   T7

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNDGG008

   Plasmid

   #231312

   Purpose

   Vector part for BEVA; BEVA2.0 Position 4 sacB sucrose counterselection module, AmpR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 4 sacB sucrose counterselection module, AmpR.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPEY-Z3-Kan

   Plasmid

   #232102

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZH61 pLIB-8His3C-spt4

   Plasmid

   #231649

   Purpose

   express S.cerevisiae spt4 in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   spt4 (SPT4 Budding Yeast)
   

   Use

   Tags

   8His-HRV3C

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPEY-Z3-Hyg

   Plasmid

   #232101

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCas9-EcRT-Z3-Nat

   Plasmid

   #232094

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS316-SSD1-L1374

    Plasmid

    #226278

    Purpose

    Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SSD1 (SSD1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAM201

    Plasmid

    #227660

    Purpose

    pRS305 3XFLAG-HRV3C-Rpn5-VTENKIF

    Depositor

    Andreas Martin

    Article

    Greene et al Elife. 2019 Nov 28;8:e49806. doi: 10.755

    Insert

    rpn5 (RPN5 Budding Yeast)
    

    Use

    Tags

    3x-FLAG-HRV3C-

    Expression

    Yeast

    Mutation

    (amino acids 121-131) VTENKIF mutated to AAAAAAA

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAM72

    Plasmid

    #227622

    Purpose

    pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

    Depositor

    Andreas Martin

    Article

    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    His-Prescission

    Expression

    Bacterial

    Mutation

    1-179 and aa 2-239, G77P

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAM315 pACYC-His6-Rpn10[ΔUIM]

    Plasmid

    #226348

    Purpose

    Expression of yeast Rpn10 with the UIM deleted

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    rpn10 (RPN10 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    delta UIM

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS315-SEC22-NCYC110

    Plasmid

    #226273

    Purpose

    Plasmid expressing the SEC22 allele from NCYC110, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS315-SEC22-L1374

    Plasmid

    #226271

    Purpose

    Plasmid expressing the SEC22 allele from L-1374, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS315-SEC22-UWOPS872421

    Plasmid

    #226272

    Purpose

    Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS315-SEC22-S288C

    Plasmid

    #226270

    Purpose

    Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SEC22 (SEC22 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML107-PMR1

    Plasmid

    #226264

    Purpose

    Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    PMR1 gRNA (PMR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS316-SSD1-NCYC110

    Plasmid

    #226279

    Purpose

    Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    SSD1 (SSD1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJJB1559

    Plasmid

    #218590

    Purpose

    Express peroxisomal Idi1p, Erg20(F96W,N127W)p, and GESp to convert IPP to geraniol with cytosolic Erg20 homodimer

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    GES
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Erg20(F96W, N127W), Erg20-Erg20 synthetic homodim…

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 300_pETcon_SARS2_FLip

    Plasmid

    #222230

    Purpose

    yeast surface display of the SARS-CoV-2 FLip variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

    Insert

    SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 297_pETcon_SARS2_Omicron-BA286

    Plasmid

    #222231

    Purpose

    yeast surface display of the SARS-CoV-2 BA.2.86 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

    Insert

    SARS-CoV-2 BA.2.86 RBD (S SARS-CoV-2 virus, Budding Yeast)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 299_pETcon_SARS2_EG-5

    Plasmid

    #222232

    Purpose

    yeast surface display of the SARS-CoV-2 EG.5 variant RBD

    Depositor

    Tyler Starr

    Article

    Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

    Insert

    SARS-CoV-2 EG.5 RBD (S SARS-CoV-2 virus, Budding Yeast)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEST17-ScChd1[Y137A]

    Plasmid

    #221198

    Purpose

    ScChd1-aa118-1274 chromatin remodeler with Y137A mutation

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    ScChd1 (aa118-1274)
    

    Use

    Tags

    6xHis-tag (Precission protease cleavable)

    Expression

    Bacterial

    Mutation

    Y137A

    Promoter

    T7

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-28a (+)-Gal4-p53

    Plasmid

    #171076

    Purpose

    Expresses Gal4-p53 fusion protein in bacteria

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4 DBD (NEWENTRY Budding Yeast)
    

    Use

    Tags

    His and p53(1-85)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RT-4

    Plasmid

    #220443

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (PUF3 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BS-TRP1-PGAL-GFP-Cps1

    Plasmid

    #207030

    Purpose

    Expression of GFP-Cps1 under GAL promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Cps1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKL1-100

    Plasmid

    #219408

    Purpose

    Reporter gene (4 operators) for zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    (42-10 op)x4-minCyc1-Venus-tCyc1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKL1-422

    Plasmid

    #219410

    Purpose

    Reporter gene (1 operator) for zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    (42-10 op)x1-minCyc1-Venus-tCyc1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKL1-424

    Plasmid

    #219412

    Purpose

    Reporter gene (3 operator) for zinc finger activator

    Depositor

    Ahmad Khalil

    Article

    Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

    Insert

    (42-10 op)x3-minCyc1-Venus-tCyc1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge