We narrowed to 50,294 results for: ANG

1. pAC154-dual-dCas9VP160-sgExpression

   Plasmid

   #48240

   Purpose

   Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA-Tag, HA-tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A H840A

   Promoter

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Daxx/pEGFP

   Plasmid

   #119021

   Purpose

   Express GFP-Daxx in mamalian cells

   Depositor

   Xiaolu Yang

   Article

   Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

   Insert

   death-domain associated protein (Daxx) (DAXX Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-egfp-U3-Osp.gRNA

   Plasmid

   #166034

   Purpose

   Empty backbone for expression of gRNA-expressing integration deficient lentiviral particles.

   Depositor

   Yujia Cai

   Article

   Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBUN421

   Plasmid

   #62204

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   maize codon optimized

   Promoter

   TaU3 and Ubi

   Available since

   Feb. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJJGL001

   Plasmid

   #180605

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   10x His and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC-fFuCas9-HTBNLS-hph

   Plasmid

   #121092

   Purpose

   Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

   Depositor

   Xiao-Jun Ji

   Article

   Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

   Insert

   Cas9 and hygromycin resist gene
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   gpdA;trpC

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNCS-CyOFP

   Plasmid

   #74278

   Purpose

   Constitutive expression of CyOFP in bacteria

   Depositor

   Michael Lin

   Article

   Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   CyOFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-SUMO-MjDim1

   Plasmid

   #189936

   Purpose

   Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq.

   Depositor

   Chuan He

   Article

   Hu et al Nat Biotechnol. 2022 Mar 14. pii: 10.103

   Insert

   rsmA (MJ_RS00455 Methanocaldococcus jannaschii)
   

   Use

   Tags

   6x His tag, FLAG tag, SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC152-dual-dCas9VP64-sgExpression

   Plasmid

   #48238

   Purpose

   Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA Tag, HA-Tag, and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Promoter

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDestTol2CG2-mnx1:ReaChR-citrine

    Plasmid

    #169046

    Purpose

    Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    ReaChR-Citrine
    

    Use

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    Promoter

    3xMnx1

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-MTS-G3635A-L17-Q2L7-T2176N-UGI-Puro

    Plasmid

    #209647

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-Q2L7-T2176N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LVDP-CArG-RE-GPR

    Plasmid

    #89762

    Purpose

    Quantitative measurement of CArG response element activity.

    Depositor

    Stelios Andreadis

    Article

    Mistriotis et al Stem Cells. 2017 Jan;35(1):207-221. doi:

    Insert

    6X CArG-RE
    

    Use

    Lentiviral

    Tags

    CMV-ZsGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid

    #178043

    Purpose

    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC93-pmax-dCas9VP160

    Plasmid

    #48225

    Purpose

    dCas9VP160 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-HA-Nrxn1α-LNS3

    Plasmid

    #128107

    Purpose

    Express HA tagged Nrxn1α-LNS3

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS3 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-HA-Nrxn1α-LNS1

    Plasmid

    #128105

    Purpose

    Express HA tagged Nrxn1α-LNS1

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS1 (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-U6sgp53-U6sgbbSapI-GFAPCre

    Plasmid

    #100275

    Purpose

    AAV-CRISPR mediated genome editing in glia / astrocytes

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

    Type

    Empty backbone

    Use

    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ265E9

    Plasmid

    #213472

    Purpose

    Gateway entry clone (attL1 & attR5) TadDE-32aa linker-zSpCas9-D10A-2xUGI for C-T and A to G base editing

    Depositor

    Yiping Qi

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Insert

    TadDE-32aa linker-zSpCas9-D10A-2xUGI
    

    Use

    CRISPR; Gateway compatible tadde-32aa linker-zspc…

    Tags

    NO

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AXBE

    Plasmid

    #204605

    Purpose

    Mammalian expression of adenine transversion base editor AXBE

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    S. pyogenes Cas9 (D10A)

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro

    Plasmid

    #209646

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-Q2L7-T2176C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge