We narrowed to 19,551 results for: Cre

1. pYSD024

   Plasmid

   #212910

   Purpose

   Encodes the wildtype S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappWT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   MFalpha1 (MF(ALPHA)1 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYSD042

   Plasmid

   #212912

   Purpose

   Encodes the S. cerevisiae HSP150 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   HSP150 (HSP150 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYSD005

   Plasmid

   #212902

   Purpose

   Encodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   KSH1 (KSH1 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYSD044

   Plasmid

   #212914

   Purpose

   Encodes the S. cerevisiae 142 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SCW4 (SCW4 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYSD022

   Plasmid

   #212908

   Purpose

   Encodes an engineered S. cerevisiae MFalpha1 pre-pro- signal peptide (MFappOPT) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   MFalpha1 (MF(ALPHA)1 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYSD009

   Plasmid

   #212906

   Purpose

   Encodes S. cerevisiae SRL1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SRL1 (SRL1 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDestTol2pA ins:TDimer mVenus

   Plasmid

   #210520

   Purpose

   Expresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Tandem dimer mVenus
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Promoter

   Zebrafish insulin promoter

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDestTol2pA ins:R198P mVenus

   Plasmid

   #210519

   Purpose

   Expresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Apollo-NADP+
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Tags

   mVenus

   Mutation

   R198P

   Promoter

   Zebrafish insulin promoter

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDestTol2pA ins:Apollo-NADP+ mVenus

   Plasmid

   #210518

   Purpose

   Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Apollo-NADP+
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Tags

   mVenus

   Promoter

   Zebrafish insulin promoter

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RS02060_pMQ30_KO_Kan

    Plasmid

    #185391

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS02060 upstream flanking homology region
    RR42_RS02060 downstream flanking homology region
    

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. RS28935_pMQ30_KO_Kan

    Plasmid

    #185392

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS28935 upstream flanking homology region
    RR42_RS28935 downstream flanking homology region
    

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RS18965_pMQ30_KO_Kan

    Plasmid

    #185394

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS18965 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS18965 upstream flanking homology region
    RR42_RS18965 downstream flanking homology region
    

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RS18970_pMQ30_KO_Kan

    Plasmid

    #185395

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS18970 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS18970 upstream flanking homology region
    RR42_RS18970 downstream flanking homology region
    

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFB_ROSA26_SpCas9_gRNAs

    Plasmid

    #174703

    Purpose

    Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

    Depositor

    Beverly L. Davidson

    Article

    Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

    Insert

    SpCas9 dual gRNAs
    

    Use

    AAV

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRISPR-StAR4GN (Lenti)

    Plasmid

    #222693

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-dual-CRISPR-U6-H1(pBP43)

    Plasmid

    #218930

    Purpose

    The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6 and H1

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRISPR-StAR4 (Retro)

    Plasmid

    #222690

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Mutation

    PGK without BbsI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRISPR-StAR4BN (Lenti)

    Plasmid

    #222692

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. XRE-pNL1.3[secNLuc]

    Plasmid

    #182295

    Purpose

    Secreted Luciferase reporter for AhR activity

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

    Insert

    Xenobiotic Response Element (CYP1A1 Human)
    

    Tags

    secNluc

    Expression

    Mammalian

    Promoter

    XRE

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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