We narrowed to 5,898 results for: dna plasmids

1. pPD1023 TRE3GV DsRedExpress2 in TU5

   Plasmid

   #139257

   Purpose

   TRE3GV promoter and DsRedExpress2 in TUPV5

   Depositor

   Joshua Leonard

   Insert

   DsRedExpress2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3GV

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CoV2-M-IRES-E

   Plasmid

   #177938

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoV2-N-B.1.1.7

   Plasmid

   #177958

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
   

   Expression

   Mammalian

   Mutation

   D3L,R203K,G204R,S235F

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CoV2-Spike-EF1a-D614G-N501Y

   Plasmid

   #177939

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (D614G, N501Y) (S )
   

   Expression

   Mammalian

   Mutation

   D614G, N501Y

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRH-1346 U6-B2M sgRNA Gag-pol v2

   Plasmid

   #201917

   Purpose

   Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-pol
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Expression

   Mammalian

   Promoter

   CAG and Human U6

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR_Hu_PRC1(WT)

   Plasmid

   #136349

   Purpose

   Human PCR1 cDNA in Gateway pENTR

   Depositor

   Guillermo de Cárcer

   Insert

   PRC1 (PRC1 Human)
   

   Use

   Gateway entry vector

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR P2R-P3-mKate2

   Plasmid

   #48345

   Purpose

   Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   mKate2
   

   Use

   Gateway entry vector

   Mutation

   Contains a stop codon at the 3' end of the c…

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR P2R-P3-SV40 polyA signal

   Plasmid

   #48627

   Purpose

   Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   SV40 early polyadenylation signal cassette
   

   Use

   Gateway entry vector

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR P4-P1R-ECFP

    Plasmid

    #48352

    Purpose

    Contributes the coding sequence for ECFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR P4-P1R-EYFP

    Plasmid

    #48350

    Purpose

    Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EYFP
    

    Use

    Gateway entry vector

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR P4-P1R-mKate2

    Plasmid

    #48344

    Purpose

    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Anna Planas, Tomas Santalucia

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    mKate2
    

    Use

    Gateway entry vector

    Mutation

    It contains a Kozak sequence but no stop codon.

    Promoter

    none

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Zahra78-79

    Plasmid

    #23960

    Depositor

    Lawrence Cohen

    Insert

    VSP (tpte Zebrafish)
    

    Tags

    Cerulean and Citrulline

    Expression

    Mammalian

    Mutation

    R153Q T156R

    Available Since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOME0276M

    Plasmid

    #182614

    Purpose

    Reversed sequence of Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome. TO_7 Control for pOME0275

    Depositor

    Dirk Dittmer

    Insert

    Reverse compliment HHV8 TO_7 control
    

    Expression

    Mammalian

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOME0237M

    Plasmid

    #182599

    Purpose

    Reverse complement sequence of anti-PAN pOME0236 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.

    Depositor

    Dirk Dittmer

    Insert

    Reverse compliment of anti-PAN (pOME0236)
    

    Expression

    Mammalian

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-Snapin

    Plasmid

    #118742

    Purpose

    Expresses EGFP tagged Snapin

    Depositor

    Beverly Koller

    Insert

    Snapin cDNA (Snapin Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRD182 (Roc1b)

    Plasmid

    #14823

    Depositor

    Robert Duronio

    Insert

    Roc1b (Roc1b Fly)
    

    Tags

    Flag

    Expression

    Bacterial

    Available Since

    July 30, 2007

    Availability

    Academic Institutions and Nonprofits only

18. HA-CPI-17-wt

    Plasmid

    #100772

    Purpose

    Mammalian expression of CPI-17

    Depositor

    Joseph Gordon

    Insert

    CPI-17 (PPP1R14A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-CPI-17-T38A

    Plasmid

    #100774

    Purpose

    Mammalian expression of CPI-17 neutral mutant

    Depositor

    Joseph Gordon

    Insert

    PPP1R14A (PPP1R14A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T38A

    Promoter

    CMV

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-EGFP-Snapin

    Plasmid

    #118743

    Purpose

    Expresses EGFP tagged Snapin

    Depositor

    Beverly Koller

    Insert

    Snapin cDNA (Snapin Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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