We narrowed to 29,111 results for: ple

1. SnFR-gamma2

   Plasmid

   #165495

   Purpose

   Green fluorescent glutamate reporter fused to TARP gamma-2 for mammalian expression.

   Depositor

   Andrew Plested

   Article

   Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

   Insert

   iGluSnFR extracellular domain fused to Stargazin (gamma-2) via NETO2 TM domain (Cacng2 Rat, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211845

   Purpose

   Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Inserts

   LRR-NEL
   GAL4-VP16-GR
   

   Use

   Synthetic Biology

   Tags

   HA, Myc, and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   6xUAS-35Smini and pUBQ10

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJZC638

   Plasmid

   #62283

   Purpose

   Expresses MCP-VP64 and dCas9 in Yeast cells

   Depositor

   Stanley Qi, Wendell Lim

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   MCP-VP64
   dCas9
   

   Use

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Nuclease activity has been deactivated by mutatio…

   Promoter

   pAdh and pGal10

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9-CAT

   Plasmid

   #80323

   Purpose

   Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Inserts

   Cas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mutation

   Promoter

   TgTUB1

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK285 (Stag-3FLAG-Bsr)

   Plasmid

   #72801

   Purpose

   C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   Stag-3FLAG-Bsr
   

   Use

   CRISPR

   Tags

   Stag-3FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMflET-o4

   Plasmid

   #101314

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   ereB resistance gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSKA413

   Plasmid

   #80381

   Purpose

   Constitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.

   Depositor

   Mustafa Khammash

   Article

   Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

   Inserts

   ccaS (complement)
   ccaR (complement)
   gfpmut3
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   FLAG tag

   Expression

   Bacterial

   Mutation

   Promoter

   ccaR and cpcG2Δ59

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-syn-SnFR-gamma8-minWPRE

   Plasmid

   #165498

   Purpose

   AAV for glutamate reporter fused to TARP gamma-8

   Depositor

   Andrew Plested

   Article

   Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755

   Insert

   iGluSnFR extracellular domain fused to TARP gamma-8 via NETO2 TM domain (Cacng8 Rat, Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PTPN13_PDZ_4

    Plasmid

    #103922

    Purpose

    Protein expression and purification of PTN13 PDZ4 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PTPN13 (PTPN13 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF6 VPS51-V5-6His

    Plasmid

    #175502

    Purpose

    V5 tagged VPS51 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

    Insert

    VPS51 (VPS51 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    A661V- please see depositor comments

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-MBP-TEVpCS-ADGRA3 CTF-FLAG

    Plasmid

    #217680

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA3 (ADGRA3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-737

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-MBP-TEVpCS-ADGRA2 CTF-FLAG

    Plasmid

    #217679

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA2 (ADGRA2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-746

    Promoter

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC5-dual-dCas9VP48-sgTetO

    Plasmid

    #48237

    Purpose

    Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9VP48 and sgTetO
    

    Use

    CRISPR

    Tags

    HA-tag and VP48

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pafG-CBE-3T

    Plasmid

    #220374

    Purpose

    for hA3A-nCas9 and gRNA expression in Aspergillus flavus

    Depositor

    Xue Gao

    Article

    Zhao et al J Am Chem Soc. 2023 Jan 11;145(1):413-42

    Inserts

    hA3A-nCas9
    tRNA and gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ptef1 and tRNA

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTU387

    Plasmid

    #178981

    Purpose

    pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    CRISPR array from Candidatus Scalindua brodae
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDRf1-4CL5-AtSCT (JBEI-11557)

    Plasmid

    #87937

    Purpose

    Co-expression in S.cerevisiae of 4CL5 and AtSCT

    Depositor

    Dominique Loque

    Article

    Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

    Inserts

    AtSCT
    4CL5 (4CL5 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSN061

    Plasmid

    #101725

    Purpose

    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-MBP-TEVpCS-ADGRL4 CTF-FLAG

    Plasmid

    #217709

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL4 (ADGRL4 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-406

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-MBP-TEVpCS-ADGRB2 CTF-FLAG

    Plasmid

    #217682

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB2 (BAI2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-911

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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