We narrowed to 78,848 results for: TRI

1. pcDNA3.1+FLAG-KrasnatG12D

   Plasmid

   #206842

   Purpose

   To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Glycine 12 to Aspartic Acid

   Promoter

   Available since

   Oct. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-Coff/Fon-GCaMP6M

   Plasmid

   #137121

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcBac1-Mb-Fluoro-Phe D6

   Plasmid

   #197577

   Purpose

   Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

   Inserts

   Mb Pyl Fluoro-Phe B5 synthetase
   Pyl-tRNA (4 copies)
   

   Use

   Tags

   Nuclear export sequence + FLAG

   Expression

   Mammalian

   Mutation

   N311A, C313M, V366G, W382T

   Promoter

   CMV and U6/H1

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-Con/Fon-sRGECO

   Plasmid

   #137126

   Purpose

   Intersectional viral expression of sRGECO in cells expressing both Cre AND Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-sRGECO
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   E217D

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-MIOS(1-875) in pRK5

   Plasmid

   #184561

   Purpose

   CMV-driven expression of an N-terminally Flag-tagged MIOS (core subunit of GATOR2) — native sequence for transient expression in mammalian cells.

   Depositor

   Kacper Rogala

   Article

   Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

   Insert

   MIOS (MIOS Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDB-His-MBP-mGSDMD

   Plasmid

   #123365

   Purpose

   Expression of full-length mouse GSDMD in E. coli

   Depositor

   Hao Wu

   Article

   Orning et al Science. 2018 Nov 30;362(6418):1064-1069

   Insert

   GSDMD (Gsdmd Mouse)
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GC52 pEGFPN1 anti-Gephyrin nanobody

   Plasmid

   #135224

   Purpose

   Mammalian expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Gephyrin (Human) recombinant llama nanobody GC52 (GPHN L. glama (llama))
   

   Use

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-Coff/Fon-iC++-EYFP

   Plasmid

   #137157

   Purpose

   Intersectional viral expression of iC++-EYFP in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-iC++-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   nEF

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-Ef1a-Coff/Fon-BFP

   Plasmid

   #137131

   Purpose

   Intersectional viral expression of BFP in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R619-M63-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166010

    Purpose

    mammalian expression of SARS-CoV-2 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) (S )
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcBac1-Ma-sfGFP 150TAG

    Plasmid

    #197568

    Purpose

    Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. JWW-1 human chimeric antibody

    Plasmid

    #66748

    Purpose

    Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

    Depositor

    Richard Roden

    Article

    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

    Inserts

    JWW-1 Heavy Chain Gamma
    JWW-1 Light Chain Kappa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    First 3 amino acids of rat light chain constant r…

    Promoter

    mEF1 and rEF1

    Available since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-Con/Fon-BFP

    Plasmid

    #137129

    Purpose

    Intersectional viral expression of BFP in cells expressing both Cre AND Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PA-DAD-wt

    Plasmid

    #53095

    Purpose

    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

    Depositor

    Ronen Zaidel-Bar, Klaus Hahn

    Article

    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

    Insert

    LOV(wt)-6aa-DAD
    

    Use

    Tags

    6x His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    L47F and C49S in Venus

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pScalps_Puro_mTet2 catalytic domain

    Plasmid

    #79554

    Purpose

    Expression of catalytic domain of mouse Tet2

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Tet2 (Tet2 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    aa990-1912

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-Ef1a-Coff/Fon-GCaMP6F

    Plasmid

    #137124

    Purpose

    Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-GCaMP6F
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R619-M64-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 D614G

    Plasmid

    #166011

    Purpose

    mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) (S )
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV51

    Available since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-EF1a-DIO-FLPo-Myc

    Plasmid

    #124641

    Purpose

    AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    FLPo
    

    Use

    AAV and Cre/Lox

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L1-Fc

    Plasmid

    #15124

    Depositor

    Vance Lemmon

    Article

    Fransen et al Hum Mol Genet. 1998 Jun . 7(6):999-1009.

    Insert

    L1-Fc (L1CAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Chimeric molecule consisting of the Fc region of …

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pBabe 3XFLAG-wtGATA6-3XAU1 puro

    Plasmid

    #72607

    Purpose

    Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pC-SUR-YR

    Plasmid

    #61768

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Sur gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Tags

    Expression

    Mutation

    Promoter

    Magnaporthe oryzae ILV promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SS80 pEGFPN1 anti-SAPAP2 nanobody

    Plasmid

    #135228

    Purpose

    Mammalian expression of anti-SAPAP (Rat) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-SAPAP2 (Rat) recombinant llama nanobody SS80 (Dlgap2 L. glama (llama))
    

    Use

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-GFP-EphrinB3-Flag(A227Y)

    Plasmid

    #155013

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    A227Y

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

    Plasmid

    #181956

    Purpose

    Genetically encoded FRET-based cAMP indicator fused to AKAP79.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
    

    Use

    Tags

    AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Ef1a-Coff/Fon-mCherry

    Plasmid

    #137134

    Purpose

    Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-EF1a-FDIO-ArchT-GFP

    Plasmid

    #124640

    Purpose

    AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    ArchT
    

    Use

    AAV; Flp/frt

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-Ef1a-Con/Foff 2.0-mCherry

    Plasmid

    #137133

    Purpose

    Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pD2_miniluc_newP7

    Plasmid

    #174105

    Purpose

    adding the mini luciferase and mini promoter to the MPRA lenti construct

    Depositor

    Paul Khavari

    Article

    Guo et al Nat Genet. 2023 Nov;55(11):1876-1891. do

    Insert

    mini luciferase mini-promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAcBac1-Mb-Fluoro-Phe B5

    Plasmid

    #197576

    Purpose

    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

    Inserts

    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    Nuclear export sequence + FLAG

    Expression

    Mammalian

    Mutation

    N311G, C313A

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. VAPA/B scFv [N479/107]

    Plasmid

    #206742

    Purpose

    Mammalian Expression of VAPA/B scFV. Derived from hybridoma N479/107 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    VAPA/B (Rattus norvegicus) recombinant scFV (Vapa Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge