We narrowed to 10,563 results for: CHL

1. pSR322

   Plasmid

   #125116

   Purpose

   Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   uhpA(REC)-ydfI(DBD)131
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSR40.15

   Plasmid

   #125082

   Purpose

   Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   ompR(REC)-ccaR(DBD)137
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSR40.29

   Plasmid

   #125088

   Purpose

   Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   ompR(REC)-ccaR(DBD)139
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTet02

   Plasmid

   #78284

   Purpose

   mRFP1 expressed behind an ATc-inducible construct

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   Tet inducible mRFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMiNGo11-3

   Plasmid

   #126058

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   mitochondria Rieske iron sulphur protein (1-100 aa)
   ccdB cassette (BsaI)
   GFP11x3 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMiNGo11-7

   Plasmid

   #126057

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   mitochondria Rieske iron sulphur protein (1-100 aa)
   ccdB cassette (BsaI)
   GFP11x7 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FB012

   Plasmid

   #119709

   Purpose

   Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   PtrpC:hph:Ttub
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AEB-No-aptamer-control -- AEB3mRFP1

   Plasmid

   #63851

   Purpose

   This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene, using a weak AEB3 promoter. It should be used when testing AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 expression.

   Depositor

   Howard Salis

   Article

   Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

   Insert

   No-aptamer control mRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   AEB3

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEM-Cas9noSsrA

   Plasmid

   #102285

   Purpose

   Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   removed ssrA tag

   Promoter

   pTet

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMpGWB419

    Plasmid

    #68684

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMpGWB420

    Plasmid

    #68685

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMpGWB422

    Plasmid

    #68687

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSALECT-Efnb2

    Plasmid

    #59707

    Purpose

    pSALECT with Efnb2 insert

    Depositor

    Christopher Anderson

    Article

    Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

    Insert

    Efnb2 (Efnb2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. cBEST4

    Plasmid

    #234660

    Purpose

    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

    Depositor

    Mingzi M. Zhang

    Article

    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

    Inserts

    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3 and tcp830

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-B/c

    Plasmid

    #227962

    Purpose

    Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    B/c
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMDC32B-B/c

    Plasmid

    #227963

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAX1_HS_gpN:SnoopTag

    Plasmid

    #225195

    Purpose

    Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SnoopTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAX1_Z11 gpN:SpyTag

    Plasmid

    #225196

    Purpose

    Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAX1_HS∆tum

    Plasmid

    #225198

    Purpose

    Used to create a markerless deletion of the E. coli HS tum gene

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    tum homology arms
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAX1_HS_lexA(Ind-)

    Plasmid

    #225199

    Purpose

    Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    lexA homology arms
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    lexA G85D

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAX1_HS_gpN:SpyTag

    Plasmid

    #225193

    Purpose

    Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAX1_HS_gpN:SpyTag003

    Plasmid

    #225194

    Purpose

    Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag003
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

    Plasmid

    #229819

    Purpose

    A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CP3

    Plasmid

    #223335

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-miniTurboID-EGFP-3HA
    

    Use

    Tags

    miniTurboID-EGFP-3HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CP2

    Plasmid

    #223334

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-EGFP-TurboID-3HA
    

    Use

    Tags

    EGFP-TurboID-3HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CP1

    Plasmid

    #223333

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-TurboID-EGFP-3HA
    

    Use

    Tags

    TurboID-EGFP-3HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CP4

    Plasmid

    #223336

    Purpose

    Mapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells

    Depositor

    Xiaohong Zhu

    Article

    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1

    Insert

    Toc64-EGFP-miniTurboID-3HA
    

    Use

    Tags

    EGFP-miniTurboID-3HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. dCas9 fused to scFv (anti-spike)

    Plasmid

    #186421

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
    

    Use

    CRISPR

    Tags

    6His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTE5418_pMBP-GlcR

    Plasmid

    #221192

    Purpose

    Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    glcR (pden4400 from Paracoccus denitrificans)
    

    Use

    Tags

    10x His tag with E. coli maltose binding protein …

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and T7 promoter-lacO

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGST-WzzE

    Plasmid

    #196662

    Purpose

    Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    TEV-cleavable GST

    Expression

    Bacterial

    Mutation

    Valine insertion at position 2.

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHis-mCherry-WzzE

    Plasmid

    #196663

    Purpose

    Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Valine insertion at position 2 of WzzE.

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPglL-strep-mCherry

    Plasmid

    #196664

    Purpose

    Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    PglL
    

    Use

    Tags

    TEV-cleavable distrep tag II and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRAR051

    Plasmid

    #185029

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 47-51 and 80-84 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 47-51 & 80-84 mutated rpmE 5'…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRAR052

    Plasmid

    #185030

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 56-61 and 68-73 mutated, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 56-61 and 68-73 mutated in rpmE 5…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRAR064

    Plasmid

    #185031

    Purpose

    400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides of rpmE 5'UTR scrambled, Only fi…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRAR074-control-sfGFP

    Plasmid

    #185032

    Purpose

    J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides of rpmE 5'UTR scrambled, Only fi…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRAR102

    Plasmid

    #185033

    Purpose

    400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    U54C mutation of rpmE 5'UTR, Only first 90 n…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRAR106

    Plasmid

    #185036

    Purpose

    400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G79U mutation of rpmE 5'UTR, Only first 90 n…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRAR116

    Plasmid

    #185040

    Purpose

    400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    U58C and U70C of rpmE of 5'UTR, Only first 9…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRAR017-L31-sfGFP

    Plasmid

    #185024

    Purpose

    400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Only first 90 nt of rpmE coding present

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRAR039

    Plasmid

    #185025

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 1-31 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 1-31 deleted from rpmE 5'UTR, On…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pRAR040

    Plasmid

    #185026

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 35-46 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 35-46 deleted from rpmE 5'UTR, O…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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43. pRAR041

    Plasmid

    #185027

    Purpose

    400 nt l31 promoter region, l31 5'UTR with nt. 47-54 & 76-86 deleted, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 47-54 & 76-86 deleted from rpmE …

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr

    Plasmid

    #218766

    Purpose

    encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

    Insert

    EcTyrRS-VSMA*
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y37V, D167G, D182S, F183M, L186A, D265R

    Promoter

    tacI

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

    Plasmid

    #218767

    Purpose

    encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

    Insert

    EcTyrRS-pAAFRS-9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y37G, L71V, D182C, F183Y, L186C, D265R

    Promoter

    tacI

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pDEST17-His10-DDX5(kf)-SNAP

    Plasmid

    #166152

    Purpose

    Expression of N. furzeri DDX5 in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5
    

    Use

    Tags

    His10 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBCC086

    Plasmid

    #202213

    Purpose

    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Gentamicin selectable marker and far-red-FP fluorescent marker mPlum

    Depositor

    Paul Schulze-Lefert

    Article

    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

    Insert

    pBCC25, pBCC33, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBCC091

    Plasmid

    #202218

    Purpose

    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

    Depositor

    Paul Schulze-Lefert

    Article

    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

    Insert

    pBCC25, pBCC30, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pBCC100

    Plasmid

    #202226

    Purpose

    Level 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Kanamycin selectable marker and far-red-FP fluorescent marker mPlum

    Depositor

    Paul Schulze-Lefert

    Article

    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

    Insert

    pBCC25, pBCC32, pBCC55, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCF702

    Plasmid

    #209650

    Purpose

    Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

    Depositor

    Bastian Molitor

    Article

    Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

    Inserts

    Downstream homologous region
    thermostable neomycin phosphotransferase gene
    Upstream homologous region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge