We narrowed to 7,286 results for: 11

1. 374 pME-ets1a

   Plasmid

   #49000

   Purpose

   full length coding sequence for zebrafish ets1a gene in gateway middle entry vector

   Depositor

   Nathan Lawson

   Article

   Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

   Insert

   v-ets erythroblastosis virus E26 oncogene homolog 1a (ets1 Zebrafish)
   

   Use

   Gateway middle entry

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-mR2-ANLN_sgRNA

   Plasmid

   #183876

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ANLN sgRNA spacer (ANLN Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB3034(X-3 MarkerFree)

   Plasmid

   #73272

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-SHP1C-ires-hCD8

   Plasmid

   #183720

   Purpose

   Expresses the myc-tagged catalytic domains of wild-type SHP-1.

   Depositor

   Takeshi Tsubata

   Article

   Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

   Insert

   SHP-1
   

   Use

   Retroviral

   Mutation

   catalitic domain alone

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pODN-AAVS1-mR2-CAAX

   Plasmid

   #183871

   Purpose

   Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. YFP-Stim1-ΔCTD

   Plasmid

   #186621

   Purpose

   Encodes Stim1 with C-terminal domain deletion fused to YFP

   Depositor

   Gia Voeltz

   Article

   Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

   Insert

   Stromal Interaction Molecule 1 (STIM1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal domain 601 to 685

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK0_017

   Plasmid

   #123932

   Purpose

   Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   BxBI attB tagBFP destination vector
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xEcoLeuT(CUA)_EF1_AnapRS

   Plasmid

   #174894

   Purpose

   AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AnapRS
   

   Expression

   Mammalian

   Mutation

   L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-GP-2-DNMT3A PWWP

   Plasmid

   #59696

   Purpose

   Contains histone modification interacting domain (DNMT3A PWWP)

   Depositor

   Albert Jeltsch

   Article

   Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

   Insert

   DNMT3A PWWP (Dnmt3a Mouse)
   

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Sept. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pONSY-CoNMM:mCherry

    Plasmid

    #111878

    Purpose

    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTK0_015

    Plasmid

    #123930

    Purpose

    Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB Destination - Insulator + tagBFP
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CytERM-mAvicFP1

    Plasmid

    #129510

    Purpose

    Expresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    CytERM-mAvicFP1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-GP-2-CBX7 Chromo

    Plasmid

    #59695

    Purpose

    Contains histone modification interacting domain (CBX7 Chromo)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    CBX7 Chromo (CBX7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK3_009

    Plasmid

    #123724

    Purpose

    Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PHYB-VP16-NLS
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV Puro SCRIB P305L

    Plasmid

    #88887

    Purpose

    SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Mutation

    Proline to Leucine mutation @ 305th amino acid

    Promoter

    MSCV promoter

    Available Since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB2899(X-2 MarkerFree)

    Plasmid

    #73271

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459-HypaCas9-mR2-RHOA_sgRNA

    Plasmid

    #183877

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    RHOA sgRNA spacer (RHOA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

    Plasmid

    #140205

    Purpose

    Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Inserts

    mCherry
    sgRNA E1
    

    Use

    CRISPR and Synthetic Biology; Proof-of-concept te…

    Mutation

    G174D

    Promoter

    PelcA and U3

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB2903(XI-2 MarkerFree)

    Plasmid

    #73275

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWay21-MKP-1 ASA

    Plasmid

    #13479

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1ASA (Dusp1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Arginine 53 to Alanine, Changed Arginine…

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only