We narrowed to 58,468 results for: tra

1. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

   Plasmid

   #174644

   Purpose

   Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
   

   Tags

   Venus-SSPB and iLID-mCherry

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NKP66 pEGFP-N2 (1176)

   Plasmid

   #62039

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

   Insert

   MARCHV (MARCHF5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV Δ18

   Plasmid

   #169465

   Purpose

   Encodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBJG1 T7-8xHis-OGT K842M

   Plasmid

   #154272

   Purpose

   Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   Lysine 842 changed to Methionine- bacterial codon…

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ratiometric FPX sensor for ERK kinase activity

   Plasmid

   #60974

   Purpose

   For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   ddRFP A-WW domain-ERK substrate-ddFP B
   

   Tags

   ERK substrate is before ddFP copy B, WW domain is…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. LV-indLS2

   Plasmid

   #123068

   Purpose

   Lentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.

   Depositor

   Kevin Brindle

   Article

   Rodriguez et al J Pathol. 2014 Mar;232(4):449-57. doi: 1

   Inserts

   CreERT2 with intron
   Firefly luciferase
   mStrawberry
   

   Use

   Lentiviral and Luciferase; Fluorescence

   Expression

   Mammalian

   Mutation

   synthetic intron added as indicated

   Promoter

   CAGGS (after Cre mediated inversion) and CAGGS (f…

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-WIV1-CoV Δ18

   Plasmid

   #164439

   Purpose

   Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   WIV1-CoV Spike truncated to remove 18 amino acids
   

   Expression

   Mammalian

   Available Since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTwist-SARS-CoV-2 Δ18 B.1.351v2

   Plasmid

   #169463

   Purpose

   Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Δ18 B.1.1.7

   Plasmid

   #169459

   Purpose

   Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-SARS-CoV-2

    Plasmid

    #164435

    Purpose

    Encodes SARS-CoV-2 Spike Protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    SARS-CoV-2 Spike (S )
    

    Expression

    Mammalian

    Available Since

    Feb. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHDM-SARS-CoV-2

    Plasmid

    #164433

    Purpose

    Encodes SARS-CoV-2 Spike Protein for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

    Insert

    SARS-CoV-2 Spike (S )
    

    Expression

    Mammalian

    Available Since

    Feb. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFAST-BAC BAF57/SMARCE1-His

    Plasmid

    #177861

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCE1 (SMARCE1 Human)
    

    Tags

    His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gfp-rt-vasa

    Plasmid

    #167322

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    rt vasa 3'UTRs (ddx4 rainbow trout)
    

    Expression

    Bacterial

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV hSyn GFP-Fxr1

    Plasmid

    #112732

    Purpose

    AAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoter

    Depositor

    Martin Beaulieu

    Article

    Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119.

    Insert

    Fxr1p (Fxr1 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    hSYN

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTwist-SARS-CoV-2 Δ18 B.1.351v3

    Plasmid

    #169464

    Purpose

    Encodes SARS-CoV-2 B.1.351v3 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v3 Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

    Plasmid

    #123194

    Purpose

    binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
    

    Use

    Luciferase

    Expression

    Plant

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV/myc/ER-αT2ib

    Plasmid

    #110769

    Purpose

    Expresses ER intrabody against mouse and human TLR2

    Depositor

    Thomas Böldicke

    Article

    Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

    Insert

    intrabody against mouse and human TLR2
    

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBJG1 T7-8xHis-OGT D554N

    Plasmid

    #154279

    Purpose

    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    Aspartate 554 changed to Asparagine- bacterial co…

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTwist-SARS-CoV-2 Δ18 B.1.351v1

    Plasmid

    #169462

    Purpose

    Encodes SARS-CoV-2 B.1.351v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.351v1 Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2-CMV/TetO2-EYFP-GW

    Plasmid

    #153309

    Purpose

    Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Yellow Fluorescent Protein

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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