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pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII

Plasmid

#209292

Purpose

Expresses R2Bm-Cas9(H840A) fusion in E. coli

Depositor

Feng Zhang

Article

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2

Tags

His, MBP, SpCas9(H840A), StrepII, and bdSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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mCHOP10(dLZ).pCDNA1

Plasmid

#21900

Depositor

David Ron

Article

Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

Insert

CHOP10 (Ddit3 Mouse)

Expression

Mammalian

Mutation

The coding region of the murine CHOP-10 cDNA was …

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only
pSB115 - pL2_pSB90_fLUC-I_tGFP-I

Plasmid

#123190

Purpose

binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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MO70 - Orai1 EC-Flag

Plasmid

#79590

Purpose

Transient expression and retroviral Expression

Depositor

Yousang Gwack

Article

Srikanth et al J Biol Chem. 2010 Feb 12;285(7):5066-75.

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

FLAG (inserted between TM3 and TM4 of the protein…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
NET50 pmRFP-N2 (489)

Plasmid

#61983

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET50 (DHRS7 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
NET97 pmRFP-N2 (641)

Plasmid

#61994

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

AADACL1 (NCEH1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

Plasmid

#123188

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF1304

Plasmid

#144293

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZMIZ2 (ZMIZ2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCLASP_Crz1(19A)_CLASP_RGS2membrane

Plasmid

#133085

Purpose

Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

Crz1*-CLASP

Expression

Bacterial and Yeast

Promoter

pADH1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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NET37 pmRFP-N2 (450)

Plasmid

#61980

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET37 (KIAA1161 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
sTR060

Plasmid

#177370

Purpose

Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

Trigger RNA

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NES-tdTomato-LINuS-WPRE

Plasmid

#159894

Purpose

AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

Depositor

Tatsushi Onaka

Article

Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

Insert

NES-tdTomato-LINuS

Use

AAV

Expression

Mammalian

Promoter

EF1α promoter

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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TAPBPL pEGFP-C3 (1765)

Plasmid

#62055

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

Insert

TAPBPL (TAPBPL Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-V5-Mfsd10

Plasmid

#120238

Purpose

Expresses V5-tagged Mfsd10 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Mfsd10 (Mfsd10 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-VSVG-mScar3-10xSunTag

Plasmid

#240630

Purpose

Expresses VSV-G, C-terminally fused to mScarlet3 and 10 repeats of the SunTag (GCN4) peptide, to luminally tag VSV-G-induced extracellular vesicles for use in EV-FUSIM assay.

Depositor

Esther Nolte-'t Hoen

Article

van den Ende et al J Extracell Vesicles. 2026 Mar;15(3):e70

Insert

VSVG-mScarlet3-10xSunTag

Tags

mScarlet3 and 10xSunTag

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Pur-ottlr3

Plasmid

#225641

Purpose

Expresses the Oncorhynchus tshawytscha toll-like receptor 3

Depositor

Bertrand Collet

Article

Collet et al Mar Biotechnol (NY). 2026 Feb 27;28(2):4

Insert

Toll-like receptor 3 (tlr3 Oncorhynchus tshawytscha)

Expression

Mammalian

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231360

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231361

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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