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pUASP dsRed Rab5 DN

Plasmid

#44823

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

S43N (dominant negative)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab11 WT

Plasmid

#37711

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab5 CA

Plasmid

#37702

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q88L (constitutive active)

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab5 DN

Plasmid

#37694

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S43N (dominant negative)

Promoter

GAL4

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab10 CA

Plasmid

#37710

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q68L (constitutive active)

Promoter

GAL4

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab8 CA

Plasmid

#37707

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab8 DN

Plasmid

#37706

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab8 (Rab8 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T22N (dominant negative)

Promoter

GAL4

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab6 CA

Plasmid

#37698

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab6 (Rab6 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q71L (constitutive active)

Promoter

GAL4

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab6 DN

Plasmid

#37697

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab6 (Rab6 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T26N (dominant negative)

Promoter

GAL4

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab3 WT

Plasmid

#37687

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab3 (Rab3 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pTMB341_ZFcharm_Prnp_SCR

Plasmid

#220844

Purpose

AAV genome expressing ZFcharm targeting Prnp; scrambled self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX307-MERS-CoV-3CL-C148A

Plasmid

#160283

Purpose

Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C148A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-NE2m-IRES-mCherry-CAAX

Plasmid

#208690

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUFlip-floxed2A-mRFP-; gcry1:BFP -0

Plasmid

#173886

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

Plasmid

#170318

Purpose

Expresses FLAG and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-rTetO-EGFP-T2A-RPL22-3xHA

Plasmid

#170326

Purpose

Tetracycline-inducible expression of HA and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Tox2-IRES-eGFP

Plasmid

#165428

Purpose

Expresses TOX2 in mammalian cells

Depositor

Patrick Hogan, Anjana Rao

Article

Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

Insert

TOX2 (Tox2 Mouse)

Tags

3xflag 5' of Tox2

Expression

Mammalian

Promoter

5' LTR

Available Since

March 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

Plasmid

#138144

Purpose

Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PguCas13b

Use

Lentiviral

Tags

NES

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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LLP249 pGK-dCas9-DNMT3a V3

Plasmid

#100938

Purpose

dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)

Use

CRISPR and Lentiviral

Tags

3xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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