We narrowed to 4,734 results for: Drosophila

1. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

   Plasmid

   #218862

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8m
   

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

   Plasmid

   #217510

   Purpose

   KD Recombinase dependent conditional smGdP-10XV5 cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
   

   Tags

   smGdP-10XV5

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHD-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199203

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int
   

   Expression

   Insect

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pExpreS2-1-C3-10H-SIII

   Plasmid

   #175444

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Tags

   3C-10His-TwinStrep and BiP Secretion Peptide

   Expression

   Insect

   Promoter

   Fused Actin-HSP70

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

   Plasmid

   #218863

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8m
   

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217515

   Purpose

   Encodes a LexAoP controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-26b-NbVHH05-HA-His

   Plasmid

   #171567

   Purpose

   Bacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-HA-His (UBE2J1 Human)
   

   Tags

   HA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHes1(467)-luc

   Plasmid

   #41723

   Depositor

   Raphael Kopan, Ryoichiro Kageyama

   Article

   Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

   Insert

   Hes1 Promoter (-467 to +46) (Hes1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Contains the murine Hes1 promoter (-467 to +46)

   Promoter

   Hes1 promoter fragment (-467 to +46)

   Available Since

   March 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW-Nb127D01-GFP

   Plasmid

   #171571

   Purpose

   Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-GFP (CXCR2 Human)
   

   Tags

   GFP

   Expression

   Insect

   Promoter

   fly actin5C promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pExpreS2-1-C3-PA-10H-SIII

    Plasmid

    #175443

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Tags

    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAW-NbVHH05-GFP

    Plasmid

    #171570

    Purpose

    Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cells

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-GFP (UBE2J1 Human)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    fly actin5C promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAW-NbVHH05-mCherry

    Plasmid

    #171572

    Purpose

    Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    NbVHH05-mCherry (UBE2J1 Human)
    

    Tags

    mCherry

    Expression

    Insect

    Promoter

    fly actin5C promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. R2pH-LAMPx3ALFA

    Plasmid

    #225136

    Purpose

    R2pH-LAMP1-labelled lysosomes with 3xLFA

    Depositor

    Massimiliano Stagi

    Article

    Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

    Insert

    Lamp1 (Lamp1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

    Plasmid

    #62951

    Purpose

    Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    3XGal80-FLAG/HA/EGFP-Hsp70
    

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHD-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199204

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHes1(467 RBPj(-))-luc

    Plasmid

    #43805

    Depositor

    Ryoichiro Kageyama

    Article

    Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

    Insert

    Hes1 Promoter (-467 to +46) RBPj(-) (Hes1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes1 promoter (-467 to +46), …

    Promoter

    Hes1 promoter fragment (-467 to +46) (RBPj-)

    Available Since

    April 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pAW-Nb127D01-mCherry

    Plasmid

    #171573

    Purpose

    Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-mCherry (CXCR2 Human)
    

    Tags

    mCherry

    Expression

    Insect

    Promoter

    fly actin5C promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218866

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19_Ntag_BbsI-Mammalian

    Plasmid

    #186674

    Purpose

    N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Mammalian N-terminal Tag
    

    Tags

    3xFlag-3xHA

    Expression

    Mammalian

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAW-CD8-Nb127D01-GFP

    Plasmid

    #171577

    Purpose

    Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    CD8-Nb127D01-GFP (CXCR2 Human)
    

    Tags

    CD8 and GFP

    Expression

    Insect

    Promoter

    fly actin5C promoter

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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