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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 21,788 results for: his

Showing: 6151 - 6180 of 21788 results

  1. FnRPR8-10

    Plasmid
    #182570
    Purpose
    Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant
    Depositor
    Timothy Springer
    Article
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 8-10
    Use
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1374A, P1376A, R1379A
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182570-plasmid-map-sequence-id-357184

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  9. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid
    #179528
    Purpose
    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 R1333A/R1335A
    Use
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed arginines 1333 and 1335 to alanine
    Promoter
    Available since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179528-plasmid-map-sequence-id-351744

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  13. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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  16. p5A1-T

    Plasmid
    #92017
    Purpose
    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with 3 TEV cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Available since
    Jan. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    92017-plasmid-map-sequence-id-201852

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  18. p5C3-T

    Plasmid
    #92022
    Purpose
    Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting sucA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    92022-plasmid-map-sequence-id-190017

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  22. BirA in pET28a (w400-2)

    Plasmid
    #26624
    Depositor
    Eric Campeau
    Insert
    BirA
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    26624-plasmid-map-sequence-id-228306

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  23. PA0288_WT_pET21a

    Plasmid
    #29793
    Depositor
    SeWon Suh
    Insert
    3-guanidinopropionase (gpuA P. aeruginosa)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2012
    Availability
    Academic Institutions and Nonprofits only

  24. PA1421_M161Y_pET21a

    Plasmid
    #29791
    Depositor
    SeWon Suh
    Insert
    Guanidinobutyrase (gbuA P. aeruginosa)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    M161Y
    Promoter
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  25. PA0288_Y157M_pET21a

    Plasmid
    #29794
    Depositor
    SeWon Suh
    Insert
    3-guanidinopropionase (gpuA P. aeruginosa)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Y157M
    Promoter
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  26. pCDB179

    Plasmid
    #91960
    Purpose
    empty expression vector for 10His-SUMO tag protein purification
    Depositor
    Christopher Bahl
    Type
    Empty backbone
    Use
    Tags
    10-His and Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    91960-plasmid-map-sequence-id-221552

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  27. pNIC-CH

    Plasmid
    #26117
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Opher Gileadi
    Type
    Empty backbone
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Sept. 17, 2010
    Availability
    Academic Institutions and Nonprofits only
    26117-plasmid-map-sequence-id-12262

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  28. pCGMbX

    Plasmid
    #42300
    Depositor
    Steven Almo
    Type
    Empty backbone
    Use
    Tags
    BiP signal sequence, His6, and V5-epitope
    Expression
    Insect
    Mutation
    Promoter
    metallothonein
    Available since
    April 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42300-plasmid-map-sequence-id-57104

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  29. pQE30-rSyntenin1

    Plasmid
    #89428
    Purpose
    bacterial expression of rat Syntenin1
    Depositor
    Fritz Rathjen
    Insert
    SDCBP
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    89428-plasmid-map-sequence-id-177625

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  30. MtPT4-eGFP

    Plasmid
    #117095
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    MtPT4-eGFP
    Use
    Tags
    His
    Expression
    Yeast
    Mutation
    Promoter
    AOX
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117095-plasmid-map-sequence-id-224993

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Showing: 6151 - 6180 of 21788 results