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We narrowed to 6,189 results for: cas9 expression plasmid

Showing: 6181 - 6189 of 6189 results

  2. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  5. pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

    Plasmid
    #209787
    Purpose
    Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)
    Depositor
    Yuxuan Guo
    Article
    Yang et al Circulation. 2024 May 28;149(22):1778-17
    Insert
    TNT4, TadA8e, SpRY N, inteinN, miR122 TS
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    TNT4
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209787-plasmid-map-sequence-id-416840

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  6. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

    Plasmid
    #207355
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Article
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207355-plasmid-map-sequence-id-408416

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  7. Multiplex-CRISPR-miR-200FAM-T2A-GFP

    Plasmid
    #179856
    Purpose
    to delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200b
    Depositor
    Brendan Jenkins
    Article
    Yu et al Oncogene. 2021 Oct 19. pii: 10.1038/s413
    Insert
    hsa-miR-200 family (miR-200b, miR-200a, miR-429, miR-141 and miR-200c ) (MIR141, MIR200B, MIR429, MIR200C, MIR200A Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179856-plasmid-map-sequence-id-357409

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  8. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Expression
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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Showing: 6181 - 6189 of 6189 results