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Showing: 4121 - 4140 of 4753 results

  3. G15-CASE

    Plasmid
    #168126
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at E244/N245 within GNA15
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168126-plasmid-map-sequence-id-332871

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  4. Gi2-CASE

    Plasmid
    #168121
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI2, GNB1, GNG2 (GNAI2, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at C112/E115 within GNAI2
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168121-plasmid-map-sequence-id-332861

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  5. TRUPATH Triple (bidirectional) GasS

    Plasmid
    #196055
    Purpose
    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196055-plasmid-map-sequence-id-385866

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  6. G13-CASE

    Plasmid
    #168127
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F125/D126 within GNA13
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168127-plasmid-map-sequence-id-332872

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  7. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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  8. Gi1-CASE

    Plasmid
    #168120
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A121/E122 within GNAI1
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168120-plasmid-map-sequence-id-332858

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  9. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168122-plasmid-map-sequence-id-332862

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  10. Gq-CASE

    Plasmid
    #168125
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG9
    Expression
    Mammalian
    Mutation
    NLuc is inserted at F124/E125 within GNAQ
    Promoter
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168125-plasmid-map-sequence-id-332869

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  14. pB-CuR-hSOD1

    Plasmid
    #232477
    Purpose
    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-CuO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232477-plasmid-map-sequence-id-471678

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  15. pB-TR-hSOD1

    Plasmid
    #232478
    Purpose
    Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TetO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232478-plasmid-map-sequence-id-471680

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  16. pMT1219

    Plasmid
    #228301
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Synthetic, Severe acute respiratory syndrome-related coronavirus 2)
    Use
    Synthetic Biology
    Tags
    GGG linker followed by His6 and MF_ secretion sig…
    Expression
    Yeast
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228301-plasmid-map-sequence-id-457688

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Showing: 4121 - 4140 of 4753 results