We narrowed to 17,201 results for: puro

1. pSuperCRISPR-sgRNA-RL69-TATT

   Plasmid

   #215849

   Purpose

   This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-RL69 with TATT stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSuperCRISPR-sgRNA-EGFP12-WT(TTTT)

   Plasmid

   #215856

   Purpose

   This plasmid encodes sgRNA that target EGFP with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-EGFP12 with TTTT stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSuperCRISPR-sgRNA-RL69-TTTC

   Plasmid

   #215855

   Purpose

   This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-RL69 with TTTC stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSuperCRISPR-sgRNA-RL69-TCTT

   Plasmid

   #215853

   Purpose

   This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-RL69 with TCTT stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSuperCRISPR-sgRNA-RL69-TTCT

   Plasmid

   #215854

   Purpose

   This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

   Depositor

   Kumiko Ui-Tei

   Article

   Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

   Insert

   sgRNA-RL69 with TTCT stretch
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMVS184

   Plasmid

   #176294

   Purpose

   Reporter_ZIFUAS_minCMVpromoter_GFP_AAVS1_Puromycin

   Depositor

   Max Staller

   Article

   Staller et al Cell Syst. 2022 Apr 20;13(4):334-345.e5.

   Insert

   Promoter and GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   4x Zinc finger binding sites and mini CMV promoter

   Available since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVS1-P-MCS

   Plasmid

   #80488

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-CT3G-CERP2-MG

   Plasmid

   #175503

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

   Plasmid

   #182306

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   NFIA (NFIA Human)
   SOX9 (SOX9 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Piggybac

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-Bi-directional

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRDA_887

    Plasmid

    #201164

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRDA_886

    Plasmid

    #201162

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSCV JMJD3 mutant

    Plasmid

    #21214

    Depositor

    Paul Khavari

    Article

    Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1390A

    Promoter

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Ki67-mCherry

    Plasmid

    #183748

    Purpose

    Expresses EGFP-Ki67-mCherry

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-TA-ERP2

    Plasmid

    #80477

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-TAC-ERP2

    Plasmid

    #80478

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAVS1-P-CAG-DEST

    Plasmid

    #80490

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMaCTag-P23

    Plasmid

    #120034

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Halo
    

    Use

    Pcr template

    Tags

    Halo

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMaCTag-P13

    Plasmid

    #120024

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPyCAG-mKate2-3xNLS-IRES-Pac

    Plasmid

    #183618

    Purpose

    Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

    Insert

    mKate2-3xNLS
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CLYBL_Bxb1-GA_LP_Dual_TC

    Plasmid

    #229776

    Purpose

    CLYBL targeting construct with the Bxb1-GA landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    FRT-mScarlet-attP(GA)-PuroR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBABE-Stat5a1*6

    Plasmid

    #130668

    Purpose

    Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

    Insert

    Stat5a (Stat5a Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Histidine 298 to Arginine and Serine 710 …

    Promoter

    LTR

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Halo-DNAPKcs HRD

    Plasmid

    #207088

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AsCas12a-6xNLS-E174R/S542R (pRG232)

    Plasmid

    #149723

    Purpose

    Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

    Depositor

    Junwei Shi

    Article

    Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Flag-HA-COPS6

    Plasmid

    #22542

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    COPS6 (COPS6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRDA_118

    Plasmid

    #133459

    Purpose

    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Flag-HA-JOSD1

    Plasmid

    #22547

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    JOSD1 (JOSD1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-TAG-ERP2

    Plasmid

    #80479

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PL552

    Plasmid

    #68407

    Purpose

    Donor empty vector with floxed puromycin resistance gene expression cassette

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse PGK promoter

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP-mCherry-Ki67

    Plasmid

    #183750

    Purpose

    Expresses EGFP-mCherry-Ki67

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Flag-HA-BRCC3

    Plasmid

    #22540

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    BRCC3 (BRCC3 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRDA_888

    Plasmid

    #201165

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMaCTag-P34

    Plasmid

    #120030

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xFLAG
    

    Use

    Pcr template

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMaCTag-P05

    Plasmid

    #120016

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    eGFP
    

    Use

    Pcr template

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMaCTag-P28

    Plasmid

    #120039

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHA

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    3xHA
    

    Use

    Pcr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Flag-HA-PARP11

    Plasmid

    #22556

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    PARP11 (PARP11 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. INS-2A-luciferase-2A-tdT

    Plasmid

    #159348

    Purpose

    Human INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    Luciferase-2A-tdTomato
    

    Use

    Ins locus knockin donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PB-CT3G-ERP2-MG

    Plasmid

    #175501

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCR1068

    Plasmid

    #111092

    Purpose

    BFP/puromycin marked gRNA vector. Contains sgRNA against GFP/BFP N-terminus.

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    BlpI site disrupted

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PAX6 donor plasmid

    Plasmid

    #105239

    Purpose

    Knock in H2B-GFP into the human PAX6 locus

    Depositor

    Lorenz Studer

    Article

    Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

    Insert

    PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMaCTag-P07

    Plasmid

    #124788

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge