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Plasmid#219822PurposeEF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)DepositorInsertdCas9
UseCRISPRTagsminiTurboExpressionMammalianMutationD10A/H841APromoterEF1aAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_ mito_roGFP2-Orp1
Plasmid#155042PurposeFluorescent redox reporterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsmitochondrial targeting sequence and roGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.v2.TetON_Cas13d_U6_DR
Plasmid#196727PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.DepositorInsertRfxCas13d-T2A-miRFP670nano
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterTREAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE-P48R
Plasmid#161816PurposeExpresses ABE-P48R in mammalian cellsDepositorInsertbpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describeā¦PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-LacI #1691
Plasmid#232636Purposelac repressor (LacI) which binds lacO sites; labeled with HaloTagDepositorInsertHaloTag-LacI
UseTagsNLSExpressionMammalianMutationnonePromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H2B-iRFP720
Plasmid#128961PurposeLentiviral plasmid for expression of far red iRFP720 nuclear markerDepositorInsertHuman histone H2B (H2BC21 Human)
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterhPGKAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#160389PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selectionDepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseCen/arsTagsExpressionYeastMutationPromoterEndogenous ATG8 promoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX 6P1 CDK2 (552)
Plasmid#61845Purposebacterial expression of GST CDK2 WTDepositorInsertCDK2 (CDK2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV-sig-mCherry-PDGFRTM-WPRE
Plasmid#216668PurposeExpresses membrane-bound mCherry in mammalian cellsDepositorInsertmCherry
UseTagsPDGFR Transmembrane DomainExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF274 SVAfw
Plasmid#178192PurposeThe SVA (SINE-VNTR-ALU) retroposon that causes human X-linked Dystonia Parkinsonism (XDP), within a BAC (pJazz) backbone.DepositorInsertSVA that inserts into the X-linked human TAF1 gene to cause X-linked dystonia Parkinsonism
UseLinear bac vectorTagsExpressionMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-HypaSpCas9-P2A-puro
Plasmid#106965Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Plus-H2B-EGFP
Plasmid#161776Purpose3rd generation lentiviral bicistronic vector for mammalian expression of H2B-EGFP and one gene of interestDepositorInsertH2B-EGFP from Addgene Plasmid #26790
UseLentiviral; BicistronicTagsEGFPExpressionMammalianMutationPromoterUbC and UbC promoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
M3-PAmcherry1
Plasmid#114189Purposestudying clustering of M3 receptors by superresolution microscopy (PALM)DepositorInsertMuscarin acetylcholine receptor 3 (CHRM3 Human)
UseTags3xHA and PAmCherryExpressionMammalianMutationPromoterSV40Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-C1-centrin-1
Plasmid#73331PurposeLentiviral vector encoding centrin-1 with an N-terminal EGFP tagDepositorInsertcentrin-1 (CETN1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBSJL1
Plasmid#71261PurposeE. coli-Veillonella shuttle plasmidDepositorTypeEmpty backboneUseE. coli-veillonella shuttle plasmidTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
POLG-pcDNA3.1
Plasmid#191487PurposeExpresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.DepositorInsertPOLG (POLG Human)
UseTagsV5 tag, 6X HisExpressionMammalianMutationPromoterCMV, T7Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-iCre-P2A-tdTomato-T2A-Puro
Plasmid#72255PurposeExpress iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoterDepositorInsertiCre-P2A-tdTomato-T2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterCB and chicken beta-actin + CMV enhancerAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Banshee RunxDN mCherry
Plasmid#80158Purposeretroviral expression of Runx1DN and mCherryDepositorInsertRunx1 (Runx1 Mouse)
UseRetroviralTagsHA and IRES-mCherryExpressionMammalianMutationDN--truncated version of the Runx1 protein contaiā¦PromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -