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We narrowed to 4,899 results for: phy

Showing: 4141 - 4160 of 4899 results

  1. pcDNA3.1-KozATG-dH6.2-2XG4S-actin

    Plasmid
    #73263
    Purpose
    Expresses dH6.2-actin fusion within mammalian cells. (dH6.2, FAP)
    Depositor
    Marcel Bruchez
    Article
    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.
    Insert
    KozATG-dH6.2-2XG4S-actin (ACTB Synthetic, Human)
    Use
    Tags
    The FAP and actin are fused with 2 copies of a G4…
    Expression
    Mammalian
    Mutation
    The dH6.2 FAP has one cysteine in each domain cha…
    Promoter
    CMV
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    73263-plasmid-map-sequence-id-137070

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  2. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid
    #197505
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…
    Promoter
    CMV and T7
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197505-plasmid-map-sequence-id-386783

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  4. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid
    #129048
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129048-plasmid-map-sequence-id-256223

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  5. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid
    #129041
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA1 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-hudTET1CD, SgRNA1 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129041-plasmid-map-sequence-id-256196

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  6. Fibronectin-human-mutated V1 O-Glycosylation site

    Plasmid
    #120406
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the third O-glycosylation site was mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains complete variable region with a mutation…
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120406-plasmid-map-sequence-id-233165

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  7. Fibronectin-human-mutated N-terminal (N), V1 and V2 Glycosylation sites

    Plasmid
    #120407
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which all three O-glycosylation sites were mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains complete variable region with mutations …
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120407-plasmid-map-sequence-id-232461

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  9. pBI121::chitEclipGFP:LAAQ

    Plasmid
    #240452
    Purpose
    Expression of indicator protein fusion (modified ecliptic pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. For details, see Comments.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of Chitinase signal, soluble modified ecliptic pHluorin and low affinity Aequorin (D119A)
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Ecliptic GFP (ecliptic pHluorin) (PMID 9671304); …
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240452-plasmid-map-sequence-id-485324

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  10. pBI121::chitRatioGFP:LAAQ

    Plasmid
    #240451
    Purpose
    Expression of indicator protein fusion (modified ratiometric pHluorin & low affinity Aequorin) for monitoring calcium concentrations and pH in cell walls of higher plants. For details, see Comments.
    Depositor
    Christoph Plieth
    Article
    Gao et al Plant Physiol. 2004 Mar;134(3):898-908.
    Insert
    Fusion of chitinase signal, soluble modified ratiometric pHluorin and low affinity Aequorin (D119A)
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Ratiometric GFP (ratiometric pHluorin) (PMID 9671…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available since
    Sept. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240451-plasmid-map-sequence-id-485323

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  11. AAV-hSyn-scFVDnmt3AL_bGHpA

    Plasmid
    #177348
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from Synapisin promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    human Synapsine
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177348-plasmid-map-sequence-id-353661

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  12. AAV-TRE-scFVDnmt3AL_bGHpA

    Plasmid
    #177354
    Purpose
    AAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    C-terminal domain of mouse Dnmt3l (194–415 aa), P…
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177354-plasmid-map-sequence-id-353614

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  13. AAV-TRE-scFVDnmt3A_bGHpA

    Plasmid
    #177355
    Purpose
    AAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    598–908 aa of mouse Dnmt3a
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177355-plasmid-map-sequence-id-353667

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  14. AAV-TRE-scFVtet1_bGHpA

    Plasmid
    #177357
    Purpose
    AAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editing
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Insert
    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    Use
    AAV
    Tags
    myc and scFV
    Expression
    Mammalian
    Mutation
    N terminal domain of human Tet1
    Promoter
    Tetracycline-dependent promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177357-plasmid-map-sequence-id-377038

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Showing: 4141 - 4160 of 4899 results