We narrowed to 2,603 results for: ubiquitin

1. pZH051

   Plasmid

   #47311

   Purpose

   Expresses Tsr-Venus-Ubiquitin-CI

   Depositor

   Jie Xiao

   Article

   Hensel et al Nat Struct Mol Biol. 2012 Aug;19(8):797-

   Insert

   wild type immunity region of lambda phage with tsr-venus-ub-cI
   

   Use

   Tags

   CI

   Expression

   Bacterial

   Mutation

   Promoter

   PR; PRM

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

2. p667-UBC-DYRK3-V5-miniTurbo_IDG-K

   Plasmid

   #158190

   Purpose

   Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   DYRK3-V5-miniTurbo (DYRK3 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   R293H- please see depositor comments

   Promoter

   Ubiquitin

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p663-UBC-miniTurbo-V5-CAMK1D_IDG-K

   Plasmid

   #158167

   Purpose

   Gateway destination clone of CAMK1D (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CAMK1D (CAMK1D Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

   Plasmid

   #130665

   Purpose

   Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
   

   Use

   Ae. aegypti expression

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Aedes aegypti poly-ubiquitin

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKF251

   Plasmid

   #52532

   Purpose

   Double-GFP reporter for DNA cleavage

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   Double-GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   ubiquitin

   Available since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ube2i

   Plasmid

   #31420

   Depositor

   Rachel Klevit

   Article

   Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

   Insert

   Ubiquitin Conjugating Enzyme (UBE2I Human)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pLANT-2b

   Plasmid

   #231487

   Purpose

   Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

   Depositor

   Venkat Gopalan

   Inserts

   SUMO^NIT (SMT3 Budding Yeast)
   FraB
   

   Use

   Tags

   His6 tag and His6-SUMO^NIT

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

   Promoter

   T7 promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. E547* FL USP7

   Plasmid

   #229001

   Purpose

   E547-mutant of human USP7

   Depositor

   Irina Bezsonova

   Insert

   USP7 1-546 (USP7 Human)
   

   Use

   Tags

   6xHis cleavable with thrombin

   Expression

   Bacterial

   Mutation

   E547 mutated to stop codon

   Promoter

   T7

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVUT-spGFP

   Plasmid

   #225486

   Purpose

   Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

   Depositor

   Robert Gross

   Article

   Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

   Insert

   Igκ leader and TAT peptide
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Human ubiquitin C

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid

    #214468

    Purpose

    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S14D S25D S32D T261D

    Promoter

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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