Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 10,763 results for: ESP

Clear keywords
Showing: 10401 - 10450 of 10763 results

  2. HA-IFITM3-F8-TAG-C105A

    Plasmid
    #122478
    Purpose
    expresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122478-plasmid-map-sequence-id-237731

    Enlarge

  3. HA-IFITM3-V93-TAG

    Plasmid
    #122479
    Purpose
    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Valine 93 to amber codon
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122479-plasmid-map-sequence-id-237724

    Enlarge

  4. HA-IFITM3-F8-TAG-C71A

    Plasmid
    #122476
    Purpose
    expresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122476-plasmid-map-sequence-id-237722

    Enlarge

  5. pUC19-J9E534

    Plasmid
    #103078
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    J9E534(Cas9 coding gene from Alicyclobacillus hesperidum URH17-3-68)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103078-plasmid-map-sequence-id-201718

    Enlarge

  8. pLEXm-hCD45d

    Plasmid
    #247052
    Purpose
    For mammalian expression of human CD45 domains 1 and 2 with C-terminal Maltose-Binding Protein fusion for secretion into the medium
    Depositor
    Omer Dushek
    Article
    Vankayala et al Bioconjug Chem. 2026 Feb 18;37(2):281-29
    Insert
    hCD45d1d2-MBP
    Tags
    His6 and Maltose binding protein (MBP)
    Expression
    Mammalian
    Mutation
    Domains 1 and 2 only, corresponding to residues 2…
    Promoter
    chick β-actin promoter
    Available Since
    Feb. 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    247052-plasmid-map-sequence-id-492518

    Enlarge

  9. pSFV_Nb139-NLS-VP64 (3'UTR)_RLEloop

    Plasmid
    #240256
    Purpose
    Attenuated PROTEUS vector with Nb139-VP64 transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.
    Depositor
    Gregory Neely
    Article
    Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:
    Insert
    nanobody Nb139
    Tags
    NLS-VP64
    Expression
    Mammalian
    Mutation
    VP64 = 4*VP16[aa437-447]
    Promoter
    SFV subgenomic promoter
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    240256-plasmid-map-sequence-id-487183

    Enlarge

  11. pTKIU-Tyr

    Plasmid
    #247649
    Purpose
    Expresses GFP-SUMO-Tyr-mCherry N-degron reporter driven by aTc responsive promoter, and constitutively expresses Ulp1, from KanR integrating mycobacterial plasmid
    Depositor
    Karl Schmitz
    Article
    Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do
    Inserts
    GFP-SUMO-Tyr-mCherry N-degron proteolysis dual-fluorescence reporter
    Ulp1 SUMO protease
    Use
    Mycobacterial integrating
    Tags
    3xFLAG tag, HA tag, and Myc tag
    Expression
    Bacterial
    Promoter
    PTet and PTet (co-operonic with GFP-SUMO-Ser-mChe…
    Available Since
    Dec. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    247649-plasmid-map-sequence-id-494847

    Enlarge

  12. pTKIU-Ser

    Plasmid
    #247650
    Purpose
    Expresses GFP-SUMO-Ser-mCherry N-degron reporter driven by aTc responsive promoter, and constitutively expresses Ulp1, from KanR integrating mycobacterial plasmid
    Depositor
    Karl Schmitz
    Article
    Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do
    Inserts
    GFP-SUMO-Ser-mCherry N-degron proteolysis dual-fluorescence reporter
    Ulp1 SUMO protease
    Use
    Mycobacterial integrating
    Tags
    3xFLAG tag, HA tag, and Myc tag
    Expression
    Bacterial
    Promoter
    PTet and PTet (co-operonic with GFP-SUMO-Ser-mChe…
    Available Since
    Dec. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    247650-plasmid-map-sequence-id-494846

    Enlarge

  24. pCas3-APOBEC1

    Plasmid
    #229536
    Purpose
    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229536-plasmid-map-sequence-id-458911

    Enlarge

  25. pCas3-CDA1

    Plasmid
    #229534
    Purpose
    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available Since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229534-plasmid-map-sequence-id-458891

    Enlarge

  27. L2117-FKBP-GPA-114-RH-CyOFP1

    Plasmid
    #222884
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222884-plasmid-map-sequence-id-444499

    Enlarge

  28. L2116-FRB-GPA-114-RH-CyOFP1

    Plasmid
    #222883
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-RH-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    222883-plasmid-map-sequence-id-444497

    Enlarge

  29. L2379-FKBP-GPA(V84R)-20-11S

    Plasmid
    #222901
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA(V84R)-20GS-NanoLuc11S
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222901-plasmid-map-sequence-id-441651

    Enlarge

  30. L2380-FKBP-GPA(V84R)-20-114

    Plasmid
    #222900
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA(V84R)-20GS-NanoLuc114
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222900-plasmid-map-sequence-id-441786

    Enlarge

  31. L2378-FRB-GPA(V84R)-20-11S

    Plasmid
    #222899
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-20GS-NanoLuc11S
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222899-plasmid-map-sequence-id-440196

    Enlarge

  32. L2377-FRB-GPA(V84R)-20-114

    Plasmid
    #222898
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-20GS-NanoLuc114
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    V84R in GPA
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222898-plasmid-map-sequence-id-440197

    Enlarge

  33. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

    Plasmid
    #222875
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    MSCV LTR
    Available Since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    222875-plasmid-map-sequence-id-441838

    Enlarge

  34. L2132-FKBP-GPA-114-5-CyOFP1

    Plasmid
    #222891
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222891-plasmid-map-sequence-id-440208

    Enlarge

  35. L2127-FRB-GPA-114-20-CyOFP1

    Plasmid
    #222886
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222886-plasmid-map-sequence-id-440205

    Enlarge

  36. L2129-FRB-GPA-114-5-CyOFP1

    Plasmid
    #222888
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222888-plasmid-map-sequence-id-440203

    Enlarge

  37. L2128-FRB-GPA-114-10-CyOFP1

    Plasmid
    #222887
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222887-plasmid-map-sequence-id-440200

    Enlarge

  38. L2131-FKBP-GPA-114-10-CyOFP1

    Plasmid
    #222890
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222890-plasmid-map-sequence-id-440190

    Enlarge

  39. L2130-FKBP-GPA-114-20-CyOFP1

    Plasmid
    #222889
    Purpose
    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    222889-plasmid-map-sequence-id-440187

    Enlarge

  44. JL054: pPB_NFκB::GFP-GEMINI(A)-P1N4_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

    Plasmid
    #228884
    Purpose
    PiggyBac plasmid expressing the destabilized GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter.
    Depositor
    Dingchang Lin
    Article
    Yan et al bioRxiv [Preprint]. 2025 Jul 18:2025.07.
    Insert
    GFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
    Expression
    Mammalian
    Promoter
    pNFκB; TRE
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    228884-plasmid-map-sequence-id-454316

    Enlarge

  50. pEND-351_Cacnes_dCas9_CRISPRi

    Plasmid
    #225617
    Purpose
    Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225617-plasmid-map-sequence-id-451418

    Enlarge

Showing: 10401 - 10450 of 10763 results