We narrowed to 8,906 results for: BLI;

1. pJK629

   Plasmid

   #72464

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to threonine, tyrosine…

   Promoter

   T7

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK541

   Plasmid

   #72447

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein
   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK569

   Plasmid

   #72455

   Purpose

   Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   synthetic gene with ~51 silent substitutions

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK587

   Plasmid

   #71696

   Purpose

   Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin reductase 1
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changes cysteine-138 to serine

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK525

   Plasmid

   #71601

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp97>Tyr mutant (AaPurE1fyw)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, tryptopha…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJK526

   Plasmid

   #71602

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-97 to tyrosine, tryptophan-165…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK601

   Plasmid

   #71606

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe mutant (AaPurE1-Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tyrosine-154 to phenylalanine, additional…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK698

   Plasmid

   #71616

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, tryptopha…

   Promoter

   T5

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK507

   Plasmid

   #71597

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Trp165>Phe mutant (AaPurE1fwf)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes tryptophan-34 to phenylalanine, tryptopha…

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK509

    Plasmid

    #71599

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn mutant (AaPurE1fww-H59N)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes tryptophan-34 to phenylalanine, histidine…

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIC232

    Plasmid

    #69737

    Purpose

    Retroviral expression of Bod1

    Depositor

    Iain Cheeseman

    Insert

    Bod1 (BOD1 Human)
    

    Use

    Retroviral

    Tags

    eYFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCASCADE-gltA2-udhA

    Plasmid

    #65819

    Purpose

    gltA-udhA targeting guide RNA array E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Insert

    gltA2-udhA guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induc…

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mEmerald-TPX2-M1-C-18

    Plasmid

    #62764

    Purpose

    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    TPX2M1 (TPX2 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    K313A, K314A, R315A Alanine mutagenesis

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. mEmerald-TPX2-M2-C-18

    Plasmid

    #62765

    Purpose

    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    TPX2M2 (TPX2 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    K158A, K159A, K313A, K314A, R315A Alanine mutagen…

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. 2I7C

    Plasmid

    #60124

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PY03671:MAC01H-E01:C21952
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available since

    Nov. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. 4JXM

    Plasmid

    #60085

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RRP9:JMC061-H10:C224101 (RRP9 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis

    Expression

    Mutation

    See comments

    Promoter

    T7

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pHL1948

    Plasmid

    #53046

    Purpose

    PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM10:RBS(st3):csrA

    Depositor

    Han Lim

    Inserts

    GFP
    tetR
    csrA
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and glgC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind, pConNoHindM10, and pConNoHindM12

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

18. JMC01B-H12

    Plasmid

    #58758

    Purpose

    bacterial expression of PDB 4O62

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4O62 (ZCWPW2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available since

    Aug. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pHL1947

    Plasmid

    #53045

    Purpose

    PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM8:RBS(st3):csrA

    Depositor

    Han Lim

    Inserts

    GFP
    tetR
    csrA
    

    Use

    Synthetic Biology

    Tags

    RBS(st3) and glgC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind, pConNoHindM12, and pConNoHindM8

    Available since

    May 13, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pUASp YFP Mtl WT

    Plasmid

    #52251

    Purpose

    Drosophila expression of YFP-Mtl

    Depositor

    Matthew Scott

    Insert

    Mtl (Mtl Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Promoter

    GAL4

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only