We narrowed to 13,113 results for: CRISPR-Cas9

1. pGY18

   Plasmid

   #221708

   Purpose

   Empty Cas9 vector for multiplex gene editing in fungi

   Depositor

   Kyle Pomraning

   Article

   Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

   Type

   Empty backbone

   Use

   CRISPR; Aspergillus

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJB172

   Plasmid

   #86992

   Purpose

   CRISPR/Cas9 in fission yeast using fluoride selection and targetting pil1

   Depositor

   Julien Berro

   Article

   Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

   Insert

   gRNA targeting pil1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSaGuide

   Plasmid

   #64710

   Purpose

   Cloning and expression of Staphylococcus aureus guide RNA

   Depositor

   Kiran Musunuru

   Insert

   Staphylococcus aureus guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMK

   Plasmid

   #62818

   Purpose

   Bacterial constitutive S. pyogenes Cas9 expression plasmid

   Depositor

   Sébastien Rodrigue

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-dSa-VPR

   Plasmid

   #99651

   Purpose

   Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9

   Depositor

   George Church

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VP64-p65-RTA activator

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. DS-SPcas

   Plasmid

   #48645

   Purpose

   Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   tracrRNA precursor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   proC and tracrRNA promoter

   Available since

   Dec. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. M-ST1cas

   Plasmid

   #48669

   Purpose

   Mammalian S. thermophilus #1 Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. M-NMcas

   Plasmid

   #48670

   Purpose

   Mammalian N. meningitidis Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

9. DS-NMcas

   Plasmid

   #48646

   Purpose

   Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   NM tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   NM tracrRNA promoter and proC

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. M-SPcas

    Plasmid

    #48668

    Purpose

    Mammalian S. pyogenes Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. DS-ST1cas

    Plasmid

    #48647

    Purpose

    Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9
    ST1 tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    proC

    Available since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. BCJJ344

    Plasmid

    #117503

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMLS544

    Plasmid

    #188882

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMLS546

    Plasmid

    #188883

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

    Plasmid

    #99679

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65(101-261)-RTA(125-190)

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV/Guide-mCherry

    Plasmid

    #170543

    Purpose

    Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV.5’eGFP/sgLMNA

    Plasmid

    #170546

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of LMNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDG330

    Plasmid

    #100898

    Purpose

    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUDP004

    Plasmid

    #101165

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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