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Viral Prep#52962-LVPurposeReady-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.DepositorPromoterEFS-NSTagsNoneAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only
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dCAS9-VP64_GFP (Concentrated Lentiviral Prep)
Viral Prep#61422-LVCPurposeReady-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.DepositorPromoterEF1aTagsNoneAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A (Lentiviral Prep)
Viral Prep#92385-LVPurposeReady-to-use Lentiviral Prep particles produced from Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A (#92385). In addition to the viral particles, you will also receive purified Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A plasmid DNA.DepositorPromoterTagsNoneAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)
Viral Prep#73179-LVPurposeReady-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.DepositorPromoterTagsNoneAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)
Viral Prep#92379-LVPurposeReady-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .DepositorPromoterTagsNoneAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENN496_miniCRISPRcharm_dSaCas9
Plasmid#220837Purposemini CRISPRcharm expression in mammalian cellsDepositorInsertmini CRISPRcharm
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRx-Sth1Cas9-L5
Plasmid#140993PurposeSth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in MycobacteriaDepositorInsertsSth1Cas9
TetR
L5 attP
L5 Int
KanR
UseUnspecified; Integrative in mycobactreiaTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-dCas9
Plasmid#125687PurposeGene knockdown for actinomycetesDepositorInsertstreptomyces codon optimized spdCas9, sgRNA casette
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-dCas9
Plasmid#112233PurposeLentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2DepositorInsertsdCas9
Porumycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationChanged: Aspartic acid 10 to Glycine, Histidine 8…PromoterEFS-NSAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-H
Plasmid#66187Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-H
Plasmid#66189Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-N
Plasmid#66191Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-B
Plasmid#66190Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-B
Plasmid#66188Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
All_in_one_CRISPR/Cas9_LacZ
Plasmid#74293Purpose"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screeningDepositorInsertsLacZ-alpha
Cas9
mCherry
UseCRISPRTagsHAExpressionBacterial and MammalianMutationPromoterLac promoter, SV40, and T7 promoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEND-351_Cacnes_dCas9_CRISPRi
Plasmid#225617PurposeCutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorInsertdCas9
UseCRISPR and Synthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9pUbi-N
Plasmid#106331PurposeExpression of Cas9 in plants, ZmUbi promoter, Kanamycin selectionDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…PromoterZmUbiAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-SP-Cas9 reporter
Plasmid#62733PurposeMammalian CRISPR-SP-Cas9 reporterDepositorInsertCRISPR-SP-Cas9 target seq + tdTomato (out of frame)
UseCRISPR and LentiviralTagsCRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…ExpressionMammalianMutationPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas9-ScaligD
Plasmid#125688PurposeGene knockout/in for actinomycetesDepositorInsertstreptomyces codon optimized spCas9, sgRNA casette, and ScaligD
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterermE*/tipAAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISV-CRISPR/Cas9PGmUbi
Plasmid#209189PurposeExpression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selectionDepositorInsertsCas9
DsRed(deltaB)
UseTagsNLSExpressionPlantMutationmissing BsaI site and plant-codon optimized with …PromoterCaMV 35S and GmUbi from Glycine max (L.) Merr. cv…Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only