We narrowed to 19,553 results for: Cre

1. pAAV-Tbg-CES2A-ΔC-S227A

   Plasmid

   #200560

   Purpose

   Secreted mouse CES2A S227A mutant driven by heptaocyte-specific promoter in AAV viral vector

   Depositor

   Jonathan Long

   Article

   Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

   Insert

   Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
   

   Use

   AAV

   Tags

   Flag

   Promoter

   CMV promoter

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1880

   Plasmid

   #141881

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREBZF (CREBZF Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6-3F2-mCherry

   Plasmid

   #73418

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3F2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3F2 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAWp28-v1 scaffold

   Plasmid

   #157976

   Purpose

   pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

   Depositor

   Alan Siu Lun Wong

   Article

   Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

   Type

   Empty backbone

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM6-3A2-mCherry

   Plasmid

   #73425

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3A2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P3A2 (orthogonal T7-lac variant)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM6-5F5-mCherry

   Plasmid

   #73422

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 5F5 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P5F5 (orthogonal T7-lac variant)

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCpep-E1GFP

   Plasmid

   #233884

   Purpose

   Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

   Depositor

   Francesco Cardarelli

   Article

   De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

   Insert

   Proinsulin-E1GFP (Ins2 Mouse)
   

   Expression

   Mammalian

   Mutation

   Inserted E1GFP into C-peptide of mouse Ins2 codin…

   Promoter

   CMVF

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. B-HypaR-SpCas9

   Plasmid

   #126764

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A; N692A; M694A; Q695A; H698A; amino acids 10…

   Promoter

   CBh

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2208

   Plasmid

   #141969

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREB3L2 (CREB3L2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Tbg-CES2C-ΔC-S230A

    Plasmid

    #200561

    Purpose

    Secreted mouse CES2C S230A mutant driven by heptaocyte-specific promoter in AAV viral vector

    Depositor

    Jonathan Long

    Article

    Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

    Insert

    Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)
    

    Use

    AAV

    Tags

    Flag

    Promoter

    TBG promoter

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6-1D4-mCherry

    Plasmid

    #73423

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1D4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P1D4 (orthogonal T7-lac variant)

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM6-4A6-mCherry

    Plasmid

    #73424

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4A6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P4A6 (orthogonal T7-lac variant)

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM6-1E4-mCherry

    Plasmid

    #73426

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1E4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P1E4 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM6-1B6-mCherry

    Plasmid

    #73420

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1B6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P1B6 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-3H5-mCherry

    Plasmid

    #73419

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3H5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P3H5 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and pTet

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

    Plasmid

    #216164

    Purpose

    Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    Gaussia princeps luciferase (with double mutation to improve stability)
    

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBFC0993

    Plasmid

    #231993

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with negative control (RFP-targeting) spacer
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119 and pTet

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF1108

    Plasmid

    #143254

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREBBP (CREBBP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBFC1171

    Plasmid

    #231994

    Purpose

    CRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Insert

    crRNA of dRfxCas13d
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pJEx (Jungle Express)

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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