We narrowed to 18,395 results for: tec

1. PrimeRoot.v2C-Cre

   Plasmid

   #201739

   Purpose

   For DNA insertion test in protoplast

   Depositor

   Caixia Gao

   Article

   Sun et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   ePPE,Cre
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH-nCas9-PBE

   Plasmid

   #98163

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-nCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCH49 (crRNA expression vector)

   Plasmid

   #217338

   Purpose

   CROP-seq crRNA expression vector with GFP and neo marker

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   AsDR-filler-AsDR8, GFP-neo
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-MCN-10His-mStayGold (E138D)

   Plasmid

   #211361

   Purpose

   Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

   Depositor

   Mohan Balasubramanian

   Article

   Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

   Insert

   mStayGold
   

   Use

   Tags

   10xHis-tag

   Expression

   Bacterial

   Mutation

   E138D

   Promoter

   T7

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAIDA1

   Plasmid

   #79180

   Purpose

   A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

   Depositor

   Gen Larsson

   Article

   Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

   Insert

   Adhesin Involved in Diffuse Adherence
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Tubulin-6

   Plasmid

   #56450

   Purpose

   Localization: Microtubules, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLX_311-Cas9

   Plasmid

   #96924

   Purpose

   for Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111

   Depositor

   John Doench, David Root, William Hahn

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   3xFlag and V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL1119 (pDonor)

   Plasmid

   #160732

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_174

   Plasmid

   #136476

   Purpose

   Lentiviral expression of EnAsCas12a

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   EnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9 nickase for Split-PE (pJUL2497)

    Plasmid

    #190104

    Purpose

    Expresses nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    Tags

    XTEN-bpNLS and bpNLS

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Puro Xlone-eGFP

    Plasmid

    #136936

    Purpose

    donor plasmid for inducible transgene expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    all-in-one tet-on system
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-M13-TEV-C-P2A-TdTomato

    Plasmid

    #92391

    Purpose

    produces AAV expressing Cal-light M13-TevC component and TdTomato

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    M13-TEV-C-P2A-TdTomato
    

    Use

    AAV and Synthetic Biology

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-GfaABC1D-GRAB_ATP1.0mut

    Plasmid

    #167580

    Purpose

    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCoofy55

    Plasmid

    #122007

    Purpose

    Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His10-SumoStar (Life Sensors) and Multi2: dependi…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCpf1b-sp

    Plasmid

    #122188

    Purpose

    Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH1-SaSrtA

    Plasmid

    #64973

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    T7 promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCpf1b

    Plasmid

    #122187

    Purpose

    Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

    Depositor

    Cheng-Cai Zhang

    Article

    Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJL1-CRM197-4xDQNAT

    Plasmid

    #128395

    Purpose

    In vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Diphtheria toxin
    

    Use

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    Inactivating G52E mutation

    Promoter

    T7

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Use

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. 35S-GWY-GAL4BD/pAM

    Plasmid

    #201231

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GWY-GAL4BD
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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