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pET28a-cMSP1D1

Plasmid

#199768

Purpose

Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP1D1

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Promoter

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-NM23-H1

Plasmid

#157987

Purpose

Expresses NM23-H1 in E.coli cell

Depositor

Byung Il Lee

Article

Han et al Bulletin of the Kor Chem Soc 2010; 31(5)

Insert

NM23-H1 (NME1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FaGH16A

Plasmid

#100910

Purpose

Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FaGH16A

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-RILP

Plasmid

#74600

Purpose

used for expression in E. coli and purification

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Rab-interacting lysosomal protein (RILP Human)

Use

Tags

FLAG, StrepII, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-clbK

Plasmid

#114152

Purpose

Expresses ClbK

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbK

Use

Tags

6xhis-Thrombin

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbC

Plasmid

#114145

Purpose

Expresses ClbC-nHis

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbC

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-A1

Plasmid

#114149

Purpose

Expresses ClbH-A1

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbH-A1

Use

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Contains only the A1 domain of ClbH

Promoter

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delBRCT

Plasmid

#173938

Purpose

Bacterial expression of PARP1 lacking BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delBRCT (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 374-520; V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP2

Plasmid

#71466

Purpose

Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 2 (SENP2 Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delCAT

Plasmid

#173942

Purpose

Bacterial expression of PARP1 lacking CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delCAT (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 661-1014

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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