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Plasmid#194181PurposeExpresses MPP8-delta2-441_10xHisDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationMPP8-delta2-441PromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-MPP8-delta442-860-10xHis
Plasmid#194182PurposeExpresses MPP8-delta442-860_10xHisDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationMPP8-delta442-860PromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-MPP8-delta600-728-10xHis
Plasmid#194183PurposeExpresses MPP8-delta600-728_10xHisDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationMPP8-delta600-728 (4xANK deletion)PromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:GFP1β10
Plasmid#125663PurposeBinary vector for the inducible expression of GFP1β10 fragment that can be used to detect a protein tagged with GFP11DepositorInsertGFP1β10
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:S11-GFP1β9
Plasmid#125665PurposeBinary vector for the inducible expression of S11-GFP1β9 fragment that can be used to detect a protein tagged with GFP10DepositorInsertS11-GFP1β9
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)
Plasmid#202555PurposeSynaptotagmin-1 sgRNA2 with TagBFP2DepositorInsertCRISPRi single guide RNA sequence against 5β-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5β - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)
Plasmid#202556PurposeSynaptotagmin-1 sgRNA3 with TagBFP2DepositorInsertCRISPRi single guide RNA sequence against 5β-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5β - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)
Plasmid#202554PurposeSynaptotagmin-1 sgRNA1 with TagBFP2DepositorInsertCRISPRi single guide RNA sequence against 5β-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5β -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
UseCRISPR and LentiviralTags3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2ExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-GFP1β10
Plasmid#125664PurposeBinary vector for the inducible expression of ER-targeted GFP1β10 fragment that can be used to detect a protein tagged with GFP11DepositorInsertER-GFP1β10
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-MPP8-delta55-118-10xHis
Plasmid#194180PurposeExpresses MPP8-delta55-118_10xHisDepositorInsertMPHOSPH8 (MPHOSPH8 Human)
UseTags10xHisExpressionMammalianMutationMPP8-delta55-118 (chromo domain deletion)PromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C234-E08: attB2r-3xFLAG-attB3
Plasmid#162912PurposeGateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteinsDepositorInserttriple FLAG epitope tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-S11-GFP1β9
Plasmid#125666PurposeBinary vector for the inducible expression of ER-targeted GFP1β9 fragment that can be used to detect a protein tagged with GFP10DepositorInsertER-S11-GFP1β9
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pResQ shFEN3 3XF-FEN1 wt
Plasmid#17752DepositorInsertFlap Endonuclease I (FEN1 Human)
UseLentiviral and RNAiTagsTriple FlagExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(V266E)
Plasmid#90089PurposeExpresses caspase 3 V266E mutant in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationV266EPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pResQ shFEN3 3XF-FEN1 D181A
Plasmid#17753DepositorInsertFlap Endonuclease I (FEN1 Human)
UseLentiviral and RNAiTagsTriple FlagExpressionMammalianMutationD181APromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKS_3HA_PAC
Plasmid#122564PurposeVector for endogenously tagging Giardia genes with a triple HA tag (Puromycin selection)DepositorTypeEmpty backboneUseTagshemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS_3HA_BSR
Plasmid#122567PurposeVector for endogenously tagging Giardia genes with a triple HA tag (Blasticidin selection)DepositorTypeEmpty backboneUseTags3x hemagglutinin tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
KSP_K406
Plasmid#129771PurposeExpresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assaysDepositorInsertKSP (kif11.L Frog)
UseTagsHis6ExpressionBacterialMutationTruncation at 367, fused to kinesin-1 starting Alβ¦PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pResQ shFEN3 3XF-FEN1 DeltaC
Plasmid#17754DepositorInsertFlap Endonuclease I (FEN1 Human)
UseLentiviral and RNAiTagsTriple FlagExpressionMammalianMutationDeletion of C-terminal 20 amino acids from FEN1PromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKS_3MYC_PAC
Plasmid#122566PurposeVector for endogenously tagging Giardia genes with a triple Myc tag (Puromycin selection)DepositorTypeEmpty backboneUseTags3x c-Myc tagExpressionMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only