Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 478 results for: adk

Showing: 401 - 420 of 478 results

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pDB.His.MBP.3C NLRP1 CARD G1467C

Plasmid

#164009

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD G1467C

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

G1467C

Available Since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG S1213A

Plasmid

#166804

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The S1213A mutant is deficient in FIIND autoprocessing

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S1213A

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTarget

Plasmid

#214052

Purpose

Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Target sequence

Use

Target rna plasmid

Expression

Bacterial

Promoter

trc promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHochTypeIII

Plasmid

#214039

Purpose

Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Cas10

Use

CRISPR

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

Plasmid

#163991

Purpose

Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 CARD (NLRP1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R

Plasmid

#163990

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1461R

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

Plasmid

#163988

Purpose

Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 UPA-CARD (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

M1457A

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase_PCi_PC-σ

Plasmid

#214040

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-Pcaspase-Pci-PC-σ

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R

Plasmid

#166809

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E1322R

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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PTK2B gRNA (BRDN0001148546)

Plasmid

#76386

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK2B (PTK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PTK2B gRNA (BRDN0001148298)

Plasmid

#76387

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK2B (PTK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PTK2B gRNA (BRDN0001147875)

Plasmid

#76388

Purpose

3rd generation lentiviral gRNA plasmid targeting human PTK2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PTK2B (PTK2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCi

Plasmid

#214038

Purpose

Bacterial expression plasmid for strep-tagged Pci from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCi

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPCaspase

Plasmid

#214042

Purpose

Bacterial expression plasmid for PCaspase from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase

Plasmid

#214043

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase-N-term

Plasmid

#214044

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

aa 1-153

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase-C-term

Plasmid

#214045

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase C-terminal fragment (aa 154-666) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

aa 154-666

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase_PCi

Plasmid

#214049

Purpose

Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-Pcaspase-PCi

Expression

Bacterial

Mutation

WT

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PC-σ

Plasmid

#214037

Purpose

Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PC-σ

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R

Plasmid

#166810

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

LL1193EE, P1214R

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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