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pTA-Mob 2.0

Plasmid

#149662

Purpose

Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

Depositor

Bogumil Karas

Article

Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

Type

Empty backbone

Use

Synthetic Biology; Conjugation helper plasmid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(1,20,6)-ADAR2(E488Q)

Plasmid

#124621

Purpose

Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

ADAR2(E488Q)

Use

AAV

Tags

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(1,20,6)-ADAR2

Plasmid

#124620

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with the ADAR2

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

ADAR2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD_NLS

Plasmid

#124704

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(2,100,50)-GFP

Plasmid

#124622

Purpose

Expresses adRNA(2,100,50) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(2,100,50)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(0,100,50)-GFP

Plasmid

#124623

Purpose

Expresses adRNA(0,100,50) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(0,100,50)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD_NLS

Plasmid

#124625

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA_(1,20,6)-GFP

Plasmid

#124619

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(1,20,6)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES

Plasmid

#124703

Purpose

Expresses MS2_adRNA targeting the RAB7A locus along with MCP_ADAR1 DD (E1008Q)_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E1008Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES

Plasmid

#124707

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS

Plasmid

#124705

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS

Plasmid

#124626

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD (E1008Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IKZF1-V2-V5

Plasmid

#107390

Purpose

Mammalian expression of IKZF1-V2-V5

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V2 (IKZF1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD_NES

Plasmid

#124630

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD_NES

Plasmid

#124706

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

Plasmid

#107387

Purpose

Lentiviral expression of IKZF1-V1-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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TC9pAB

Plasmid

#71310

Purpose

used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

Depositor

Karl Degenhardt

Insert

Cas9

Use

In vitro transcription

Tags

NLS

Expression

Mutation

Promoter

T7

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRD503

Plasmid

#220788

Purpose

Overexpresses HTkC in E. coli cells

Depositor

Remus Dame

Article

Schwab et al bioRxiv 2023.06.01.543357

Insert

HTkC

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promotor

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAKgfplux1

Plasmid

#14083

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAKlux2.1

Plasmid

#14081

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Bacterial luciferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge

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