Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,332 results for: pET28a

Showing: 401 - 420 of 1332 results

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pET28a-OuIAD(H514E)

Plasmid

#226972

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514E)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514E

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(H514A)

Plasmid

#226971

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(C500S)

Plasmid

#226970

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(C500S)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

C500S

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(G853A)

Plasmid

#226969

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(G853A)

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

G853A

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W4-HECT

Plasmid

#187710

Purpose

Express his-tagged NEDD4L W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 543-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-542

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W3-W4-HECT_C874only

Plasmid

#187711

Purpose

Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 with mutations (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491; mutations C702A, C776…

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyCatcher -AVI

Plasmid

#223635

Purpose

Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Tags

AVI tag and His6

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-ChmN-C47A

Plasmid

#221367

Purpose

Express C47A ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN-C47A

Tags

His6

Expression

Bacterial

Mutation

changed Cys 47 to Ala

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-4_sheep_catalytic domain

Plasmid

#214331

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-11_rat_catalytic domain

Plasmid

#214329

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Rat)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-4_lemur_catalytic domain

Plasmid

#214328

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-4_rabbit_catalytic domain

Plasmid

#214330

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEWS

Plasmid

#208799

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A; S437A

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-Δ24

Plasmid

#208801

Purpose

Xenopus laevis His6-Myc-DONSON (1-24aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

Δ24, deletion of 1-24aa

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEW

Plasmid

#208802

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-S437A

Plasmid

#208803

Purpose

Xenopus laevis His6-Myc-DONSON (S437A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Mutation

S437A

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-ΔD1

Plasmid

#208804

Purpose

Xenopus laevis His6-DONSON (1-155aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His

Expression

Bacterial

Mutation

ΔD1, deletion of 1-155aa

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-TrxF

Plasmid

#207454

Purpose

E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

Depositor

Joseph Jez, Sarah Stainbrook

Article

Stainbrook et al bioRxiv 2022.08.24.505184

Insert

Thioredoxin F

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for E coli expression

Promoter

T7

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a LZ control-FLAG

Plasmid

#199678

Purpose

Bacterial expression of Leucine Zipper (LZ) control-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper control

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED WT-FLAG

Plasmid

#199679

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED WT (GPR155 Human)

Tags

6xHis and Flag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 401 - 420 of 1332 results

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