We narrowed to 1,322 results for: pET28a

1. pET28a(+)-NEDD4L_W4-HECT

   Plasmid

   #187710

   Purpose

   Express his-tagged NEDD4L W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 543-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-542

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a(+)-NEDD4L_W3-W4-HECT_C874only

   Plasmid

   #187711

   Purpose

   Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-975 with mutations (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-491; mutations C702A, C776…

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SpyCatcher -AVI

   Plasmid

   #223635

   Purpose

   Protein purification of SpyCatcher003 (S49C) with AVI and 6xHis tags from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   AVI tag and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-FKBP-empty

   Plasmid

   #221332

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   6xHis-3xFKBP empty scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-ChmN-C47A

   Plasmid

   #221367

   Purpose

   Express C47A ChmN in E. coli

   Depositor

   Emily Balskus

   Article

   Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

   Insert

   chmN-C47A
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   changed Cys 47 to Ala

   Promoter

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_Caspase-4_sheep_catalytic domain

   Plasmid

   #214331

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_Caspase-11_rat_catalytic domain

   Plasmid

   #214329

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (Casp4 Rat)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a_Caspase-4_lemur_catalytic domain

   Plasmid

   #214328

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_Caspase-4_rabbit_catalytic domain

   Plasmid

   #214330

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-DONSON-DEWS

    Plasmid

    #208799

    Purpose

    Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    D374A; E377A; W381A; S437A

    Promoter

    T7

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-DONSON-Δ24

    Plasmid

    #208801

    Purpose

    Xenopus laevis His6-Myc-DONSON (1-24aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Δ24, deletion of 1-24aa

    Promoter

    T7

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-DONSON-DEW

    Plasmid

    #208802

    Purpose

    Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    D374A; E377A; W381A

    Promoter

    T7

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-DONSON-S437A

    Plasmid

    #208803

    Purpose

    Xenopus laevis His6-Myc-DONSON (S437A)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    S437A

    Promoter

    T7

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-DONSON-ΔD1

    Plasmid

    #208804

    Purpose

    Xenopus laevis His6-DONSON (1-155aa removed)

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    ΔD1, deletion of 1-155aa

    Promoter

    T7

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-6xHis-TrxF

    Plasmid

    #207454

    Purpose

    E. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tag

    Depositor

    Joseph Jez, Sarah Stainbrook

    Article

    Stainbrook et al bioRxiv 2022.08.24.505184

    Insert

    Thioredoxin F
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    T7

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a LZ control-FLAG

    Plasmid

    #199678

    Purpose

    Bacterial expression of Leucine Zipper (LZ) control-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper control
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a LZ LED WT-FLAG

    Plasmid

    #199679

    Purpose

    Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper LYCHOS LED WT (GPR155 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a Cdk2ap1CAN-MutTER

    Plasmid

    #178033

    Purpose

    Expression vector for the purpose of protein purification.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Nonviral

    Tags

    6xHIS - Thrombin - MBP- TEV-TRS

    Expression

    Bacterial

    Mutation

    Changed T(108)A, E(109)A, R(110)A

    Promoter

    T7LacI

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-matNODALvar-HIS

    Plasmid

    #115265

    Purpose

    For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL variant mature peptide (NODAL Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-eGFP-Pep-H

    Plasmid

    #185788

    Purpose

    Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    eGFP-Pep4
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge