We narrowed to 1,961 results for: rpe

1. PLEKHA3 (FAPP1) g2 lentiCRISPRv2-opti

   Plasmid

   #218657

   Purpose

   Knockout vector for human PLEKHA3 (FAPP1)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   PLEKHA3 (PLEKHA3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PLEKHA8 (FAPP2) g1 lentiCRISPRv2-opti

   Plasmid

   #218658

   Purpose

   Knockout vector for human PLEKHA8 (FAPP2)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   PLEKHA8 (PLEKHA8 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLEKHA3 (FAPP1) g1 lentiCRISPRv2-opti

   Plasmid

   #218656

   Purpose

   Knockout vector for human PLEKHA3 (FAPP1)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   PLEKHA3 (PLEKHA3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPLS24

   Plasmid

   #17285

   Depositor

   Nancy Craig

   Article

   Sharpe et al EMBO J. 1998 Oct 1. 17(19):5822-31.

   Insert

   TnsD (tnsD E. coli transposon Tn7)
   

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215014

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti UBC LysoFlux (HaloTag7-mGFP-Galectin3)

   Plasmid

   #215008

   Purpose

   Express a Lysophagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDEST17-UbE2R2

   Plasmid

   #15806

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2R2 (UBE2R2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215015

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHAGE pGK LysoFlux (HaloTag7-mGFP-Galectin3)

   Plasmid

   #215009

   Purpose

   Express a Lysophagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST17-UbE2D3

    Plasmid

    #15784

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    see Depositor Comments below

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist-msEEA1

    Plasmid

    #214991

    Purpose

    Gateway entry vector with mouse EEA1

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (Eea1 Mouse)
    

    Use

    Gateway

    Tags

    3xFLAG

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXDC61-LepA

    Plasmid

    #21843

    Depositor

    Howard Shuman

    Article

    Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

    Insert

    LepA (lepA L. pneumophila)
    

    Tags

    TEM

    Expression

    Bacterial

    Available Since

    Sept. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. Rab11A[8-175]-His-C Q70L

    Plasmid

    #228042

    Purpose

    Protein purification construct. Rab11 crystallography truncation with a C-terminal cys for maleimide labeling. Constitutively active mutation (Q70L).

    Depositor

    Wade Harper

    Insert

    Rab11A[8-175]-His-C Q70L (RAB11A Human)
    

    Expression

    Bacterial

    Mutation

    Q70L

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV UBC hTMEM115-3C-3xHA-P2A-eGFP

    Plasmid

    #214996

    Purpose

    Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    TMEM115 (TMEM115 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST17-Cdc34a

    Plasmid

    #18674

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Cdc34A (CDC34 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

    Plasmid

    #214997

    Purpose

    Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    TMEM115 (Tmem115 Mouse)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET15-UbE2D1

    Plasmid

    #15782

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
    

    Tags

    6x His, T7 tag, and Xpress tag (enterokinase clea…

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HC-M

    Plasmid

    #173481

    Purpose

    An rrnB P1-based GFP ATP reporter in E. coli

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

    Insert

    GFP-mut2
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Promoter

    rrnB P1

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDA303

    Plasmid

    #195199

    Purpose

    Overexpressing 6His-TetR in E. coli.

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al Front Bioeng Biotechnol. 2022 Sep 28;10:

    Insert

    TetR
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T5 promoter

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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