We narrowed to 18,363 results for: Tec

1. pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

   Plasmid

   #171435

   Purpose

   Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   Lifeact-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19/mChe-Px

   Plasmid

   #183170

   Purpose

   Construct for transient expression of peroxisomes-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-Px
   

   Use

   Tags

   10 aa peroxisome targeting signal 1 (IHHPRELSRL) …

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19/Pd-mChe

   Plasmid

   #183172

   Purpose

   Construct for transient expression of plasmodesmata-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   Pd-mCherry
   

   Use

   Tags

   AtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. JD639

   Plasmid

   #160398

   Purpose

   Binary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformation

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Insert

   35S::vitis miR396-resistant GRF4-GIF1 chimera
   

   Use

   Binary vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19/mChe-Cs

   Plasmid

   #183171

   Purpose

   Construct for transient expression of cytoskeleton-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-Cs
   

   Use

   Tags

   363 aa of A. thaliana FIMBRIN 1 (AtFIM1)

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MS2-epegRNA-Dnmt1

   Plasmid

   #211372

   Purpose

   Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MS2-epegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC19/CF-mChe

   Plasmid

   #183166

   Purpose

   Construct for transient expression of cytoplasmic foci-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   CF-mCherry
   

   Use

   Tags

   AtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19/Tp-mChe

   Plasmid

   #183165

   Purpose

   Construct for transient expression of tonoplast-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   Tp-mCherry
   

   Use

   Tags

   28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFBD-SpyTag-noro-VLP

   Plasmid

   #165989

   Purpose

   Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyTag-noro-VLP (ORF2 Synthetic, norovirus)
   

   Use

   Tags

   SpyTag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Cas9 TLS2

    Plasmid

    #196978

    Purpose

    Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9 with TLS2 tag
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pG10-90

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CD9-mEGFP

    Plasmid

    #182864

    Purpose

    Localization: Tetraspanin/Membrane

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p2CT-His-MBP-Lse_C2c2_WT

    Plasmid

    #83486

    Purpose

    Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lse_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FokI-dCas9

    Plasmid

    #52970

    Purpose

    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

    Depositor

    David Liu

    Article

    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Fok1 fused to dCas9
    

    Use

    CRISPR

    Tags

    Fok1 nuclease (homodimeric wildtype) and NLS

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JD690

    Plasmid

    #160397

    Purpose

    Binary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformation

    Depositor

    Jorge Dubcovsky

    Article

    Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

    Insert

    35S::citrus GRF4-GIF1 chimera
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BiP-mGFP

    Plasmid

    #62231

    Purpose

    Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.

    Depositor

    Erik Snapp

    Article

    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

    Insert

    hamster BiP-mGFP-KDEL
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-Trono-EFS-Blast-P2A-TurboRFP

    Plasmid

    #228893

    Purpose

    Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-GfaABC1D-GRAB_ATP1.0

    Plasmid

    #167579

    Purpose

    Express the ATP sensor GRAB_ATP1.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP sensor GRAB_ATP1.0 (P2RY1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSQT1601

    Plasmid

    #53369

    Purpose

    human codon optimized Csy4 and FokI-dCas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    hCsy4-T2A-NLS-hFokI-dCas9-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cas9 TLS1

    Plasmid

    #196977

    Purpose

    Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9 with TLS1 tag
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pG10-90

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge