We narrowed to 6,469 results for: poly

1. pGEMHE-bNup98-DN-NLS-mClover3

   Plasmid

   #171495

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   Nup98-DN (NUP98 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. p1A CbbL-

   Plasmid

   #162693

   Purpose

   p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   H. neapolitanus CbbLS and S. elongatus Prk
   

   Use

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   CbbL K194M (inactive rubisco)

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET14b-dabAB2 D353A

   Plasmid

   #133016

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Use

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Mutation

   D353A

   Promoter

   t7

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET14b-dabAB2 C351A

   Plasmid

   #133014

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Use

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Mutation

   C351A

   Promoter

   t7

   Available since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCRII apoA-Ib

   Plasmid

   #121896

   Purpose

   Plasmid for making zebrafish apoA-Ib in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoA1b (apoa1b Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PCRII apoBb.2

   Plasmid

   #121899

   Purpose

   Plasmid for making zebrafish apoBb.2 in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoBb.2 (apobb.2 Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCRII apoA-IVb.3

   Plasmid

   #121905

   Purpose

   Plasmid for making zebrafish apoA-IVb.3 in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   apoa4b.3 (apoa4b.3 Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCRII apoA-IVa

   Plasmid

   #121902

   Purpose

   Plasmid for making zebrafish apoA-IVa in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   ApoA4a (apoa4a Zebrafish)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PCRII apoA-Ia

   Plasmid

   #121895

   Purpose

   Plasmid for making zebrafish apoA-Ia in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   apoA-Ia (apoa1a Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDECKO-mCherry MALAT1_Enhancer.4

    Plasmid

    #78539

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDECKO-mCherry MALAT1_Enhancer.3

    Plasmid

    #78538

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDECKO-mCherry MALAT1_Exon.3

    Plasmid

    #78542

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDECKO-mCherry MALAT1_Enhancer.2

    Plasmid

    #78537

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and U6 (for expressing sgRNA)

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCellFree_G03 AKT1

    Plasmid

    #67102

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCellFree_G03 BMPR1A

    Plasmid

    #67105

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BMPR1A (BMPR1A Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 EHD2

    Plasmid

    #67107

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    EHD2 (EHD2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCellFree_G03 GTF2H1

    Plasmid

    #67108

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H1 (GTF2H1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. T7opt in pCAGGS

    Plasmid

    #65974

    Purpose

    Human codon-optimized T7 polymerase

    Depositor

    Benhur Lee

    Article

    Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

    Insert

    T7-opt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. p7SL-neo-TET

    Plasmid

    #51285

    Purpose

    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    7SL RNA (RN7SL2 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    7SL upstream pollII enhancer

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cas9-NAT

    Plasmid

    #64329

    Purpose

    Cas9 expression cassette carried by a yeast single-copy episome plasmid

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    Human-optimized S. pyogenes Cas9 with Clonnat marker gene expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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