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hs-FL-Kek1-myc2

Plasmid

#41170

Depositor

Norbert Perrimon

Article

Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

Insert

Kek1 (kek1 Fly)

Use

Tags

2X myc

Expression

Insect

Mutation

Promoter

Hsp70

Available since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pAAVf-EnhCB-lacZnls mir-122 1xBS

Plasmid

#35643

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

1 miR-122 target site

Use

AAV

Tags

Expression

Mutation

Promoter

CB

Available since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat S572A

Plasmid

#33319

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S571A

Promoter

SV40

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pLEX_305-C-dTAG

Plasmid

#91798

Purpose

Lentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG system

Depositor

Behnam Nabet, James Bradner

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459) V2.0

Plasmid

#62988

Purpose

Cas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_H2A_C1

Plasmid

#85053

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

H2A (H2AC16 Human)

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNEU-hMbPylRS-4xU6M15

Plasmid

#105830

Purpose

Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

Depositor

Irene Coin

Article

Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

Inserts

Humanized MbPylRS
tRNAM15

Use

Tags

Expression

Mammalian

Mutation

(Y271A, Y349F)MbPylRS

Promoter

CMV and U6

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

Plasmid

#192616

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RIboL1-jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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YAP/TAZ-TRE-mStrawberry reporter

Plasmid

#158682

Purpose

This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR1A FKBP12F36V-OGT

Plasmid

#154287

Purpose

Entry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x HA and FKBP12(F36V)

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p53-TRE-mStrawberry reporter

Plasmid

#158684

Purpose

This plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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HIF-TRE-mStrawberry reporter

Plasmid

#158681

Purpose

This plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pATZ-Myc

Plasmid

#177718

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

Myc

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-capsid protein

Plasmid

#215701

Purpose

Produces the Chikungunya virus capsid protein

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV capsid

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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VCP(wt)-EGFP

Plasmid

#23971

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Use

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

none

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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pMSCV-F-del Casp9.IRES.GFP

Plasmid

#15567

Depositor

David Spencer

Article

Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

Insert

FKBP12(V36)-p30Caspase9 (FKBP1A Human)

Use

Retroviral

Tags

FKBP12 and HA epitope

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

Promoter

Available since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pEF1α:flex-mcherry

Plasmid

#222618

Purpose

Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

Depositor

Franck Polleux

Article

Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

Insert

Cre-dependent mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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VCP(DK0)-EGFP

Plasmid

#23974

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Use

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

E305QE578Q

Promoter

Available since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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pRSF-G1TFAKRS

Plasmid

#177311

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CROPseq-iT7

Plasmid

#211699

Purpose

NIS-Seq-compatible lentiviral vector for expressing sgRNAs

Depositor

Jonathan Schmid-Burgk

Article

Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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