We narrowed to 70,757 results for: LAS

1. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

   Plasmid

   #219653

   Purpose

   General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus

   Depositor

   Thomas Oertner

   Insert

   2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
   

   Use

   AAV

   Tags

   2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)

   Expression

   Mammalian

   Mutation

   Promoter

   nEf Addgene # 149296

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

   Plasmid

   #198450

   Purpose

   Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.11_Nued2_E1_dAP1-2

   Plasmid

   #203837

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dAP1-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   KO of both AP1 sites

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWB511-TP-UnaG-FLAG

   Plasmid

   #203479

   Purpose

   For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   Plastid transit peptide fused UnaG-FLAG
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB511-TP-tagRFP

   Plasmid

   #203482

   Purpose

   For Agrobacterium transformation. tagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   TagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB602-TP-mCherry-UnaG

   Plasmid

   #203483

   Purpose

   For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   Plastid transit peptide fused mCherry-UnaG
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.11_Nued2_E1_dAP1

   Plasmid

   #203835

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dAP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   KO of AP1 upstream

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL4.11_Nued2_E1_dAP2

   Plasmid

   #203836

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dAP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   KO of AP1 downstream

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNHA-C1-HsNot3_K

   Plasmid

   #146771

   Purpose

   Mammalian Expression of HsNot3

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsNot3 (CNOT3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations compared to the sequence giv…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLNHA-C1-HsNot10_K

    Plasmid

    #146772

    Purpose

    Mammalian Expression of HsNot10

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot10 (CNOT10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutation compared to the sequence give…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLNHA-C1-HsNot2_J

    Plasmid

    #146697

    Purpose

    Mammalian Expression of HsNot2

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot2 (CNOT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGWT35S-VC155

    Plasmid

    #194051

    Purpose

    Transient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    VC155 (mVenus β-strands 8 to 11, amino acids 155−239)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWT35S-VN154

    Plasmid

    #194050

    Purpose

    Transient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    VN154 (mVenus β-strands 1 to 7, amino acids 1−154)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXD70LacZ8.1-Pcadh-LacZ

    Plasmid

    #191624

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXD70LacZ9-Phcdh-LacZ

    Plasmid

    #191625

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRISPR_HLA_DPB

    Plasmid

    #164989

    Purpose

    Expression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DPB1*01:01:01
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CRISPR_HLA_DQB

    Plasmid

    #164991

    Purpose

    Expression of gRNA targeting HLA-DQB locus, including DQB1*05:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DQB1*05:01:01
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CRISPR_HLA_DRB

    Plasmid

    #164992

    Purpose

    Expression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DRB1*01:02:01
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CRISPR_HLA_DQA

    Plasmid

    #164990

    Purpose

    Expression of gRNA targeting HLA-DQA locus, including DQA1*01:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DQA1*01:01:01
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CRISPR_HLA_DPA

    Plasmid

    #164988

    Purpose

    Expression of gRNA targeting HLA-DPA locus, including DPA1*02:01:08

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DPA1*02:01:08
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LPLKMLNI_epitope

    Plasmid

    #165015

    Purpose

    Expression of CMV UL83 (116-123) fused to IL-2 signal sequence

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV UL83 (116-123)
    

    Use

    Lentiviral

    Tags

    human IL2 signal sequence at N-term

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLNHA-C1-HsNot8_J

    Plasmid

    #146699

    Purpose

    Mammalian Expression of HsNot8

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot8 (CNOT8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAT15065-HF-CBE

    Plasmid

    #163010

    Purpose

    plasmid expressing HF-CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HF-CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAT15066-Hypa-CBE

    Plasmid

    #163011

    Purpose

    plasmid expressing Hypa-CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    Hypa-CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAT15067-HypaR661A-CBE

    Plasmid

    #163012

    Purpose

    plasmid expressing HypaR661A-CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    Hypa-CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    R661A

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAT15069-HeF-CBE

    Plasmid

    #163014

    Purpose

    plasmid expressing HeF-CBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HeF-CBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAT15000-BE4max

    Plasmid

    #163015

    Purpose

    plasmid expressing BE4max

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAT9991-eABE

    Plasmid

    #162999

    Purpose

    plasmid expressing eABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAT9992-HF-ABE

    Plasmid

    #163000

    Purpose

    plasmid expressing HF-ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    HF-ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAT9995-evoABE

    Plasmid

    #163003

    Purpose

    plasmid expressing evoABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    evoABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge