We narrowed to 32,433 results for: uros

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RabV CVS-N2c(deltaG)-APEX2-Venus

Plasmid

#73467

Purpose

Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

APEX2-Venus

Use

Neurotropic virus

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-jRGECO1a

Plasmid

#102995

Purpose

Expresses histone fused jRGECO1a under the synapsin promoter

Depositor

David Cox

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

H2B-jRGECO1a

Use

AAV

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p194-RV-CAG-dio-mScarlet-T2A-Brn2

Plasmid

#204741

Purpose

Conditional expression of target gene Brn2 with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

Brn2

Use

Retroviral

Promoter

CAG

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pOC1 Gria1-GFP-FRB KI

Plasmid

#183428

Purpose

CreOFF knock-in for GluA1-GFP-FRB (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and GFP-FRB donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-I-Sce1-slc6a3[11kb]:egfp

Plasmid

#53240

Purpose

allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

Depositor

Edward Burton

Article

Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

Insert

DAT-GFP

Use

Cloning

Tags

GFP

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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lenti SYN-SVI-dCas9-VPR

Plasmid

#164575

Purpose

Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

Insert

FLAG-dCas9-VPR containing an SV40 intron

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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p254-RV-CAG-dio-mScarlet-T2A-Dach1-202

Plasmid

#204742

Purpose

Conditional expression of target gene Dach1 with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

Dach1

Use

Retroviral

Promoter

CAG

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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p186-RV-CAG-dio-mScarlet-T2A-mVenus

Plasmid

#204740

Purpose

Conditional expression of target gene mVenus with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mVenus

Use

Retroviral

Promoter

CAG

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A D197,199N

Plasmid

#195704

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9 with D1…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-ND1-K139D-T2A-mCherry

Plasmid

#178587

Purpose

Expression of NEUROD1-K139D and mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1-K139D (NEUROD1 Human)

Use

AAV

Expression

Mammalian

Mutation

NEUROD1-K139D

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-ND1-R111L-T2A-mCherry

Plasmid

#178586

Purpose

Expression of NEUROD1-R111L and mCherry under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1-R111L (NEUROD1 Human)

Use

AAV

Expression

Mammalian

Mutation

NEUROD1-R111L

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-GFP-shRNA-scramble

Plasmid

#182503

Purpose

Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

Depositor

William Wisden

Article

Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

Insert

pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Promoter

hSyn promoter

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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p164-pAAV-mDlx-FLEX-mScarlet

Plasmid

#204689

Purpose

Fluorescent reporter under the control of the Dlx promotor

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

mScarlet palmitoylation

Expression

Mammalian

Promoter

mDlx

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-iCre-2A-venus

Plasmid

#182499

Purpose

Expresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression

Depositor

William Wisden

Article

Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

Insert

iCre-2A-Venus

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

fragment of histidine decarboxylase gene promoter…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFSynW pHluorin-SYT9 IRES mRuby3

Plasmid

#195698

Purpose

Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Use

Lentiviral

Tags

pHluorin

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt9 IRES mRuby3

Plasmid

#195700

Purpose

Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

encodes only the first 68 amino acids of Tac1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B D330, 332N

Plasmid

#195705

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9 with D3…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB D197,199,330,332N

Plasmid

#195706

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9 with D1…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW Substance P-pHluorin IRES mRuby3

Plasmid

#195699

Purpose

Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Tac1 (Tac1 Mouse)

Use

Lentiviral

Tags

pHluorin

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D197, 199N IRES GFP

Plasmid

#195701

Purpose

Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D197, 199N mutations

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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