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pcDNA5FRT/TO-Strep/HA-UBE2Q2

Plasmid

#124668

Purpose

Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2Q2 (UBE2Q2 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160S)

Plasmid

#66872

Purpose

Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)

Tags

Histag from pET30Xa-LIC

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-Fbxo7-IRES-GFP

Plasmid

#171786

Purpose

retrovirus production

Depositor

Heike Laman

Article

Lomonosov et al PLoS One. 2011;6(6):e21165. doi: 10.1371

Insert

Fbxo7 isoform 1 (FBXO7 Human)

Use

Retroviral

Mutation

none

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptger1

Plasmid

#170306

Purpose

A knock-out vector for the mouse Ptger1

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Ptger1 gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAMV:nAChRgamma-eGFP

Plasmid

#29693

Depositor

Henry Lester

Article

Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

Insert

GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma (Chrng Mouse)

Tags

AMV promotor and poly(A) tail

Expression

Bacterial

Mutation

eGFP inserted after position S380

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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p-miR-148a

Plasmid

#65052

Purpose

Expression of miR148a in mammalian cells

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

hsa-miR-148a (pre-miRNA) (MIR148A Human)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRSETa-mKikGR-V69T

Plasmid

#98574

Purpose

photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mKikGR-V69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDsRed-HP1alpha

Plasmid

#179702

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2014;78(4):55

Insert

heterochromatin protein alpha (CBX5 Human)

Tags

dsRed

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAT-mWasabi-G

Plasmid

#164170

Purpose

Expresses mWasabi under the control of TetO promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal, pAAV-TRE backbone

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mWasabi

Use

AAV

Expression

Mammalian

Promoter

tetO

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPTG-GG-sg2+sg3

Plasmid

#127199

Purpose

Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

tRNA-gRNA

Use

Empty sgrna plasmid

Expression

Mammalian

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCardinal-MannII-N-10

Plasmid

#56167

Purpose

Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MannII

Tags

mCardinal

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA-p160(1-200)

Plasmid

#43

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pSUPER.retro BRIT1

Plasmid

#16206

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

shBRIT1 (MCPH1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pZ_P-GAP-LacI-Mit1AD

Plasmid

#126722

Purpose

LacI-Mit1AD on pGAPZ_ A expression vector

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Mit1 activation domain coding sequence

Expression

Yeast

Promoter

GAP

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgs2_Balbc

Plasmid

#170301

Purpose

A knock-out vector for the mouse Ptgs2 in Balb/c

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG3_F(ab')2

Plasmid

#105860

Purpose

Expression plasmid coding for heavy chain of mouse F(ab')2 of IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG3 F(ab')2 (Ighg3 Mouse)

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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KTK_247

Plasmid

#180529

Purpose

Entry vector containing pTac promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pTac promoter

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin

Plasmid

#134866

Purpose

Expresses EGFP-tagged mutant of VAMP1

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Vesicle-associated membrane protein 1 (VAMP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 97-118

Promoter

EF1a

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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XE223 human myc-Chibby-pCS2+MT

Plasmid

#16825

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

myc-Chibby (CBY1 Human)

Use

Xenopus expression

Tags

6-myc

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

Plasmid

#63886

Purpose

An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

Depositor

Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

ER-localized GCaMP3(wt)

Use

AAV

Promoter

human SYN1

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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