We narrowed to 5,315 results for: pcdna3.1

1. PME-1 pcDNA-StrepIII

   Plasmid

   #119292

   Purpose

   Mammalian overexpression of StrepII-tagged full length PPME1

   Depositor

   Jukka Westermarck

   Article

   Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

   Insert

   PPME1 (PPME1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. VN-beta Synuclein

   Plasmid

   #89483

   Purpose

   expresses human beta Synuclein fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Tenreiro et al Hum Mol Genet. 2016 Jan 15;25(2):275-90.

   Insert

   SNCB (SNCB Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. hTPST2

   Plasmid

   #11253

   Depositor

   Hyeryun Choe

   Article

   Choe et al Cell. 2003 Jul 25. 114(2):161-70.

   Insert

   TPST2 (TPST2 Human)
   

   Use

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pC1-dmito-oROS-HT

   Plasmid

   #216418

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   dmito-oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HiBiT-FZD7

   Plasmid

   #195845

   Purpose

   Expresses human FZD7 with an N-terminal HiBiT

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD7 (FZD7 Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. β₂AR-mCFP

   Plasmid

   #38260

   Depositor

   Robert Lefkowitz

   Article

   Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

   Insert

   β₂AR (ADRB2 Human)
   

   Use

   Tags

   Flag, mCFP, and signal peptide sequence (MKTIIALS…

   Expression

   Mammalian

   Mutation

   diglycine linker (replacing terminator codon)

   Promoter

   CMV

   Available Since

   Aug. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. LUC01 (L/Sec/P)

   Plasmid

   #112144

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U)

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

   Plasmid

   #162566

   Purpose

   Used for the dual color labeling of cPLA2 and LaminB1 expression.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Inserts

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   Lamin B1 (lmnb1 Zebrafish)
   

   Use

   Tags

   EGFP, P2A, and mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SV40

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NSP3-FR-2Strep

   Plasmid

   #210315

   Purpose

   Expresses SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   NSP3 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pC1-oROS-HT-CaaX

    Plasmid

    #216417

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT-CaaX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FLAG-VSVG

    Plasmid

    #80606

    Purpose

    Mammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    VSV-G
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Use

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Perox-mTurq2-Apollo-NADP+

    Plasmid

    #193304

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Use

    Tags

    mTurqoise2, peroxisomal targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CoV2-N-T205I

    Plasmid

    #177954

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (T205I) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T205I

    Promoter

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HCMVgO-sfGFP (TB40/E)

    Plasmid

    #136451

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MAP4K3-GFP

    Plasmid

    #21731

    Depositor

    L. Miguel Martins

    Article

    Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

    Insert

    MAP4K3 (MAP4K3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IDE-2Strep

    Plasmid

    #210355

    Purpose

    Expresses IDE fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    IDE (IDE Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 HA-Sumo1 Q94P

    Plasmid

    #48965

    Purpose

    Expresses HA SUMO1 Q94P mutant in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mutated Q94P

    Promoter

    T7

    Available Since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pDisplay-oROS-HT

    Plasmid

    #216416

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    pDisplay-oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hTPST1

    Plasmid

    #11252

    Depositor

    Hyeryun Choe

    Article

    Choe et al Cell. 2003 Jul 25. 114(2):161-70.

    Insert

    TPST1 (TPST1 Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 19, 2006

    Availability

    Academic Institutions and Nonprofits only