We narrowed to 9,400 results for: Coli

1. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2

   Plasmid

   #205051

   Purpose

   Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2_mCherry(-6)-L2
   

   Use

   Tags

   GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3

   Plasmid

   #205052

   Purpose

   Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2_mCherry(-6)-L3
   

   Use

   Tags

   GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNZ18-CAH

   Plasmid

   #203159

   Purpose

   Replicates in E. coli, S. cerevisiae, and A. laidlawii

   Depositor

   Bogumil Karas

   Article

   Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

   Insert

   Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDT2

   Plasmid

   #199119

   Purpose

   circular transcription template

   Depositor

   Jeff Gelles

   Article

   Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

   Insert

   lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

   Promoter

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Atg32 [200-341] 1B

   Plasmid

   #190431

   Purpose

   Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

   Depositor

   Michael Ragusa

   Article

   Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

   Insert

   Atg32
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Atg11 [699-800] pHis2

   Plasmid

   #190264

   Purpose

   Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

   Insert

   ATG11
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. His SUMO HNRNPA1 dHexa LCD

   Plasmid

   #197014

   Purpose

   E. coli expression vector

   Depositor

   Tanja Mittag

   Article

   Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

   Insert

   HNRNPA1
   

   Use

   Tags

   6His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPEPT1

   Plasmid

   #195001

   Purpose

   Production of peptides in E. coli as GB1 fusion

   Depositor

   Marko Hyvönen

   Article

   Pantelejevs et al Chem Sci. 2023 Nov 21;14(47):13915-13923

   Type

   Empty backbone

   Use

   Tags

   His8-tag and StrepTagII-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXD70Tet(HcdR)-HcdR

   Plasmid

   #191648

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), hcdR complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   HcdR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXD70Tet(CadR)-CadR

    Plasmid

    #191649

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (TetR), cadR complementation

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CadR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7-10-QUAS-GFP_ColE1

    Plasmid

    #171657

    Purpose

    QUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-10-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. T7-15-QUAS-GFP_ColE1

    Plasmid

    #171658

    Purpose

    QUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-15-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. T7-TetO-QF_T7-LacO-TetR_p15A

    Plasmid

    #171659

    Purpose

    Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-LacO-TetR-T7 Stop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. T7-0-QUAS-GFP_ColE1

    Plasmid

    #171650

    Purpose

    QUAS directly downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-0-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. QUAS-0-T7-CcdB_ColE1

    Plasmid

    #171652

    Purpose

    QUAS directly upstream of a T7 promoter. Expresses CcdB in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    CcdB-ssrA
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. T7-5-QUAS-GFP_ColE1

    Plasmid

    #171656

    Purpose

    QUAS five base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-5-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PET28a-cpLumiLuc-amylase sensor

    Plasmid

    #186293

    Purpose

    Expression alpha-amylase sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-amylase sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PET28a-cpLumiLuc-HSA sensor

    Plasmid

    #186294

    Purpose

    Expression Human serum albumin sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-HSA sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-His6-Sumo-CylK-Strep

    Plasmid

    #185962

    Purpose

    Expresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparation

    Depositor

    Emily Balskus

    Article

    Braffman et al Elife. 2022 Feb 25;11. pii: 75761. doi:

    Insert

    CylK
    

    Use

    Tags

    His6-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPBO.ACT002

    Plasmid

    #182712

    Purpose

    aTc inducible dCasRx-IF1

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    3x(GGGS)-Escherichia Coli Initiation Factor 1

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pTet

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FAK C-term

    Plasmid

    #186147

    Purpose

    For expresseion of recombinant FAK c-terminal domain in E. coli

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSF-1b-S15-T79C

    Plasmid

    #133048

    Purpose

    Expresses tagless S15-T79C mutant ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    S15 E.coli ribosomal protein (rpsO E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T79C

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSF-1b-S3-M129C

    Plasmid

    #132949

    Purpose

    Expresses tagless S3-M129C mutant ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    E.coli ribosomal protein S3 (rpsC E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M129C

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSF-1b-S19-T48C

    Plasmid

    #128597

    Purpose

    Expresses tagless S19-T48C mutant 30S ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S19 (rpsS E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T48C

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CcCDP_p15A

    Plasmid

    #179272

    Purpose

    Expresses CcCDP in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Insert

    cellodextrin phosphorylase
    

    Use

    Synthetic Biology

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET19b-DAD2 cPGFP(A149)

    Plasmid

    #165998

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(A149)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET19b-rShHTL7 cPGFP(E167)

    Plasmid

    #166004

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(E167)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET19b-rShHTL7 cPGFP(D165)

    Plasmid

    #166003

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(D165)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET19b-rShHTL7 cPGFP(C164)

    Plasmid

    #166002

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rShHTL7 cPGFP(C164)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET19b-DAD2 cPGFP(N180)

    Plasmid

    #166000

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    DAD2 cPGFP(N180)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge