We narrowed to 11,678 results for: cel.2

1. ACTB-(A)2x

   Plasmid

   #112056

   Purpose

   ACTB mRNA tagged with 2 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   2 copies of Riboglow tag (variant A) after stop c…

   Promoter

   CMV

   Available since

   June 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJC-elav1.8kb-TALE1-VP64-P10

   Plasmid

   #104609

   Purpose

   Expresses TALE1 under the control of a 1.8kb elav enhancer element

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   1.8kb elav enhancer element (elav Fly)
   

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HOXB6 (human) FLAG pMSCV

   Plasmid

   #8534

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   Retroviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   contains improved ribosomal binding site and FLAG…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP Kind2 S159/181/666A

   Plasmid

   #105310

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/181/666A

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX Grap2-SH2

   Plasmid

   #46435

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Grap2 (GRAP2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 52-159

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-crRNA(TBK1)

   Plasmid

   #109322

   Purpose

   AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

   Depositor

   Feng Zhang

   Article

   Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

   Insert

   mCherry-KASH (TBK1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

   Plasmid

   #194226

   Purpose

   Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

   Plasmid

   #214706

   Purpose

   Mammalian expression of human CALR ins5 YD/FL-FLAG

   Depositor

   Norio Komatsu

   Article

   Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

   Insert

   CALR ins5 YD/FL-FLAG (CALR Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   MSCV

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)

   Plasmid

   #204199

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Use

   Tags

   3xHA, eGFP and T7x2

   Expression

   Mammalian

   Mutation

   Gly to Ala 317,318,341,1171

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_SEPT9_i1 S22A S23A-msfGFP

    Plasmid

    #180338

    Purpose

    mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S22A S23A

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_SEPT9_i1 R10A R15A-msfGFP

    Plasmid

    #180336

    Purpose

    mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 R10A R15A

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_SEPT9_i1 S12A S13A-msfGFP

    Plasmid

    #180337

    Purpose

    mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S12A S13A

    Promoter

    CMV

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti_CRISPRi_sgAR10

    Plasmid

    #167003

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 10 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. DHC1-mIAA17 Hygro

    Plasmid

    #140544

    Purpose

    DHC1 tagging with mIAA7

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5-LAP-MPS1-dTPR

    Plasmid

    #114048

    Purpose

    Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-dTPR (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta TPR (deletion of TPR domain 63-191)

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5-LAP-MPS1-d60

    Plasmid

    #114046

    Purpose

    Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    MPS1-d60 (TTK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta 60 (deletion of NTE domain)

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP Kind2 S159/181/666E

    Plasmid

    #105314

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/181/666E

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS2181

    Plasmid

    #158421

    Purpose

    AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple32 (CLDN5 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple32

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRY2high-mCherry-Raf1

    Plasmid

    #104064

    Purpose

    Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and Raf1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2high-mCherry-Raf1 (CRY2, RAF1 Mustard Weed, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CRY2PHR E490R

    Promoter

    CMV

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. tetO-HAND2

    Plasmid

    #170690

    Purpose

    doxycycline-inducible overexpression of HAND2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    HAND2 (HAND2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSems-leader-mEGFP-MBP-TMD(ALA7)

    Plasmid

    #193051

    Purpose

    Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    ALA7-TMD
    

    Use

    Tags

    Ig k-chain leader sequence, MBP, and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)

    Plasmid

    #145840

    Purpose

    Lentiviral vector genome to ectopically express human Ace2

    Depositor

    Caroline Goujon

    Article

    Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

    Insert

    human Ace2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. tetO-NR2F1

    Plasmid

    #170698

    Purpose

    doxycycline-inducible overexpression of NR2F1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    deletion of 36G- please see depositor comments

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEMS2230

    Plasmid

    #158420

    Purpose

    AAV plasmid with Ple253 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple253 (PITX3 )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple253

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

    Plasmid

    #182425

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-hGH (GH1 Human)
    VNp-LZ
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSH-Csy4-T2A-SpRFN

    Plasmid

    #85754

    Purpose

    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-dCas9
    

    Use

    CRISPR

    Tags

    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMK297 (DHC1-mAID Hygro)

    Plasmid

    #140542

    Purpose

    DHC1 tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMK296 (DHC1-mAID Neo)

    Plasmid

    #140541

    Purpose

    DHC1 tagging with mAID

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSH-Csy4-T2A-SaRFN

    Plasmid

    #85755

    Purpose

    Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-SadCas9
    

    Use

    CRISPR

    Tags

    3xHA tag, Csy4, and FokI fusion via 36 amino acid…

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CAG

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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