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pDONR223_NKX2-1_HD_160-223AA

Plasmid

#221477

Purpose

Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelNTerm_160-371AA

Plasmid

#221487

Purpose

NKX2-1 N-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal domain

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-hPGK_NKX2-1_Short_V5-GFP

Plasmid

#221498

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYGS Q16C

Plasmid

#217674

Purpose

For expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experiment

Depositor

Larry David

Insert

CRYGS (CRYGS Human)

Use

Tags

6XHis and SUMO

Expression

Bacterial

Mutation

Glutamine 16 changed to Cysteine

Promoter

T7

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS8

Plasmid

#218716

Purpose

Expresses N-terminally EGFP-tagged BBS8 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS8 (TTC8 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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MP6-SlugABEN-738

Plasmid

#214348

Purpose

Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugCas9-P2A-puro

Plasmid

#214353

Purpose

Expresses SlugCas9 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 and puromycin resistance genes

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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XPO6-2Strep

Plasmid

#210356

Purpose

Expresses XPO6 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

XPO6 (XPO6 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TRAM2-2Strep

Plasmid

#210374

Purpose

Expresses TRAM2 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TRAM2 (TRAM2 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TRAM1-2Strep

Plasmid

#210352

Purpose

Expresses TRAM1 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TRAM1 (TRAM1 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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SGTA-EGFP

Plasmid

#210373

Purpose

Expresses SGTA fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SGTA (SGTA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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SGTA-2Strep

Plasmid

#210353

Purpose

Expresses SGTA fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SGTA (SGTA Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro

Plasmid

#210856

Purpose

Lentiviral expression vector encoding FIP200 mutant-mCherry

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

FIP200 (RB1CC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

L324D/R543D/R548D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-L476/L479D

Plasmid

#210845

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

L476/L479D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (450-517)-A452D/F453D

Plasmid

#210851

Purpose

Mammalian expression of ATG13 (450-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

A452D/F453D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (363-450)

Plasmid

#210849

Purpose

Mammalian expression of ATG13 (363-450) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-GST-ATG13 (460-517)-F509/F512D

Plasmid

#210847

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

F509/F512D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-L495/L499D

Plasmid

#210846

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

L495/L499D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG13 (460-517)-F466D/F470D

Plasmid

#210844

Purpose

Mammalian expression of ATG13 (460-517) mutant

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13 (ATG13 Human)

Use

Tags

Expression

Mammalian

Mutation

F466D/F470D

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge