We narrowed to 6,525 results for: sas

1. R26-EGFP MMEJ donor vector

   Plasmid

   #137926

   Purpose

   R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-EGFP-bpA with micro-homology arms
   

   Use

   Mouse Targeting

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHJ18

   Plasmid

   #48357

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV_A2S-8

   Plasmid

   #188654

   Purpose

   AAV vector expressing SaAID-2S and gRNA

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Inserts

   AID2S--nSaCas9-UGI
   Sa-sgRNA
   

   Use

   AAV and CRISPR

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   Scp1 and U6

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHJ41

   Plasmid

   #48360

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHJ42

   Plasmid

   #48359

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHW1133

   Plasmid

   #229869

   Purpose

   Destination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCE

   Depositor

   Han Wang

   Article

   Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-pleCopyCatcher

   Plasmid

   #174061

   Purpose

   CopyCatcher insertion donor for the pale locus

   Depositor

   Ethan Bier

   Article

   Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

   Insert

   Pale (Pale Fly, Synthetic)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAVS1-Puro-CAG-mNG2(1-10)

   Plasmid

   #206042

   Purpose

   Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

   Insert

   AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
   

   Use

   CRISPR and Synthetic Biology; Donor template

   Expression

   Mammalian

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. OmpA-mNG-sfTq2ox

   Plasmid

   #124220

   Purpose

   Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

   Depositor

   Tanneke den Blaauwen

   Article

   Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

   Insert

   OmpA-mNG-sfTq2ox
   

   Expression

   Bacterial

   Available Since

   April 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT077

    Plasmid

    #137879

    Purpose

    Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT076

    Plasmid

    #137880

    Purpose

    Donor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. single cut NH-donor

    Plasmid

    #83575

    Purpose

    eGFP reporter donor containing single SA cutting site for NHEJ knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Nhej donor

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Sg-A

    Plasmid

    #83807

    Purpose

    Express sgRNA targeting SA site

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. (401) pAAV CMV-ZsGreen U6-gRNA

    Plasmid

    #163021

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. double cut NH-donor

    Plasmid

    #83576

    Purpose

    eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Nhej donor

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. OmpA-mNG-mCh

    Plasmid

    #124216

    Purpose

    Periplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mCh

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

    Insert

    OmpA-mNG-mCh
    

    Expression

    Bacterial

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. (326) pAAV SV40-ZsGreen U6-gRNA

    Plasmid

    #163020

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-FLAG-SBP-STAG2 (S97X)

    Plasmid

    #73964

    Purpose

    STAG2 cDNA (CCDS43990) S97X

    Depositor

    Todd Waldman

    Article

    Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

    Insert

    STAG2 (STAG2 Human)
    

    Tags

    FLAG-SBP

    Expression

    Mammalian

    Mutation

    S97X

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only