We narrowed to 9,400 results for: Coli

1. pET19b-DAD2 cPGFP(A165)

   Plasmid

   #165999

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   DAD2 cPGFP(A165)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET19b-HisDAD2 cPGFP(G164)

   Plasmid

   #165997

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   HisDAD2 cPGFP(G164)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET19b-rShHTL7 cPGFP(L166)

   Plasmid

   #165995

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   rShHTL7 cPGFP(L166)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET19b-DAD2 cPGFP(G164)

   Plasmid

   #165993

   Purpose

   Expression of coding sequence in Escherichia coli

   Depositor

   Claudia Vickers

   Article

   Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

   Insert

   DAD2 cPGFP(G164)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pACYCDuet1-tglCDEF

   Plasmid

   #134677

   Purpose

   Expresses TglC, TglD, TglE, TglF in E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Inserts

   TglC
   TglD
   TglE
   TglF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSFDuet1-tglA

   Plasmid

   #134673

   Purpose

   Expresses His6-TglA in E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Insert

   TglA
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pACYCDuet1-ammA(∆Trp56)

   Plasmid

   #134683

   Purpose

   Expresses His6-AmmA(∆Trp56) in E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Insert

   ammA(delW56)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   deletion of W56

   Promoter

   T7

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28b-tglF

   Plasmid

   #134681

   Purpose

   Expresses His6-TglF in E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Insert

   TglF
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b-GFP-tglG

   Plasmid

   #134679

   Purpose

   Expresses His6-GFP-TglG fusion in E. Coli

   Depositor

   Wilfred van der Donk

   Article

   Ting et al Science. 2019 Jul 19;365(6450):280-284.

   Insert

   TglG
   

   Use

   Tags

   His6-GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b-tglHI

    Plasmid

    #134672

    Purpose

    Expresses His6-TglH and TglI in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglH
    TglI
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAL- S. pombe TSC1 (1-431)

    Plasmid

    #132616

    Purpose

    expresses S. pombe TSC1 (1-431) in E. coli

    Depositor

    Wenqing Xu

    Article

    Sun et al Nat Commun. 2013;4:2135. doi: 10.1038/nc

    Insert

    TSC1 (1-431)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDB325

    Plasmid

    #113669

    Purpose

    expresses Ulp1_R1 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R1
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMAL-SsoPCNA3

    Plasmid

    #107069

    Purpose

    Expresses S. solfataricus PCNA3 fused to MBP in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

    Insert

    S. solfataricus PCNA3
    

    Use

    Tags

    6xHis-thrombin cleavage site and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCML 221

    Plasmid

    #98587

    Purpose

    pMS2-CYFP. Expresses MS2 protein-CYFP fusion in E. coli for TriFC assay (kanR).

    Depositor

    Lydia Contreras

    Article

    Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

    Insert

    MS2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CTL2

    Plasmid

    #92212

    Purpose

    Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

    Depositor

    Douglas Rees

    Article

    Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

    Insert

    CTL2 of PIEZO1
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAM5084

    Plasmid

    #85105

    Purpose

    Ptrc-ECFP-KaiC fusion cloned into Km resistance gene of pACYC177. To look for KaiC localization in E. coli.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    ecfp-KaiC
    

    Use

    Tags

    ECFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPD1cR

    Plasmid

    #86970

    Purpose

    Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, G108C in PCNA1

    Promoter

    T7 promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPD1R

    Plasmid

    #86969

    Purpose

    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPD2cX

    Plasmid

    #86968

    Purpose

    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…

    Promoter

    T7 promoter

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC3

    Plasmid

    #85087

    Purpose

    Expresses PCNA3 protein fused to P450cam (CYP101A1) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    PCNA3 fused to P450cam
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 36INS2-pBAD_GB

    Plasmid

    #78677

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pBAD Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 36INS2-pBAD_FB

    Plasmid

    #78676

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pBAD Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 36INS2-pBAD_EB

    Plasmid

    #78675

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pBAD Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 36INS2-pBAD_AB

    Plasmid

    #78674

    Purpose

    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]

    Depositor

    Douglas Densmore

    Article

    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

    Insert

    Insulated pBAD Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK227

    Plasmid

    #71126

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    GyrA intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDEST14-SARA

    Plasmid

    #67389

    Purpose

    Expression of native hSara in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SARA (SAR1B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pfMDH_K381A

    Plasmid

    #67732

    Purpose

    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed lysine 381 to alanine

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pfMDH_N300D

    Plasmid

    #67728

    Purpose

    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to aspartate

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pfMDH_R373A

    Plasmid

    #67731

    Purpose

    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed arginine 373 to alanine

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pfMDH_N300S

    Plasmid

    #67729

    Purpose

    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Use

    Tags

    6x-His tag

    Expression

    Bacterial

    Mutation

    Changed asparagine 300 to serine

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only