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Showing: 4261 - 4280 of 77185 results

  1. pCRISPR-mcBEST

    Plasmid
    #209416
    Purpose
    Plasmid for multiplexed cytosine base editing in streptomycetes
    Depositor
    Tilmann Weber
    Article
    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2
    Inserts
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    base editing cassette: PtipA ; sgRNAs: PkasO* an…
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209416-plasmid-map-sequence-id-416613

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  4. pGLTR-S-PURO

    Plasmid
    #58244
    Purpose
    GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance
    Depositor
    Stephan Geley
    Article
    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:
    Inserts
    attR1-Cm(R)-CcdB-attR2
    Puro(R)
    Use
    Lentiviral and RNAi; Gateway destination vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and no
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58244-plasmid-map-sequence-id-89655

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  5. pEasyG2_hph

    Plasmid
    #184915
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184915-plasmid-map-sequence-id-367948

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  6. pBY011-AMN1ko

    Plasmid
    #183099
    Purpose
    S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.
    Depositor
    Gabor Balazsi
    Article
    Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:
    Insert
    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
    Use
    Tags
    KanMX
    Expression
    Bacterial and Yeast
    Mutation
    only includes external sequences (504 bp each) as…
    Promoter
    URA3, TEF1
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183099-plasmid-map-sequence-id-361230

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  12. pC-BAR-YR

    Plasmid
    #61766
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Bar gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Tags
    Expression
    Mutation
    Promoter
    trpC promoter
    Available since
    May 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    61766-plasmid-map-sequence-id-442755

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  13. pCAP03-sp44/p21

    Plasmid
    #120232
    Purpose
    Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.
    Depositor
    Bradley Moore
    Article
    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-
    Insert
    bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    aac(3)IV (apramycin resistance gene)
    Promoter
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    120232-plasmid-map-sequence-id-233750

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Showing: 4261 - 4280 of 77185 results